Imaging intracellular RNA distribution and dynamics in living cells.

Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE

Original Publication: New York, NY : Nature Pub. Group, c2004-

Cells/*cytology ; Cells/*metabolism ; Cytophotometry/*methods ; RNA, Messenger/*analysis ; RNA, Messenger/*metabolism; Aptamers, Nucleotide/chemistry ; Cell Survival ; Fluorescent Dyes/chemistry ; Green Fluorescent Proteins/chemistry ; Green Fluorescent Proteins/genetics ; Microscopy, Fluorescence/methods ; Oligonucleotide Probes/chemistry ; RNA, Messenger/chemistry ; RNA-Binding Proteins/chemistry ; RNA-Binding Proteins/genetics ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/genetics

Powerful methods now allow the imaging of specific mRNAs in living cells. These methods enlist fluorescent proteins to illuminate mRNAs, use labeled oligonucleotide probes and exploit aptamers that render organic dyes fluorescent. The intracellular dynamics of mRNA synthesis, transport and localization can be analyzed at higher temporal resolution with these methods than has been possible with traditional fixed-cell or biochemical approaches. These methods have also been adopted to visualize and track single mRNA molecules in real time. This review explores the promises and limitations of these methods.

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MH079197 United States MH NIMH NIH HHS

0 (Aptamers, Nucleotide)
0 (Fluorescent Dyes)
0 (Oligonucleotide Probes)
0 (RNA, Messenger)
0 (RNA-Binding Proteins)
0 (Recombinant Fusion Proteins)
147336-22-9 (Green Fluorescent Proteins)

Date Created: 20090501 Date Completed: 20090526 Latest Revision: 20211020

20250114

10.1038/nmeth.1321

19404252