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Development of medium for enhanced production of glutaminase-free L-asparaginase from Pectobacterium carotovorum MTCC 1428.

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  • المؤلفون: Kumar S;Kumar S; Pakshirajan K; Venkata Dasu V
  • المصدر:
    Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2009 Sep; Vol. 84 (3), pp. 477-86. Date of Electronic Publication: 2009 Apr 08.
  • نوع النشر :
    Evaluation Study; Journal Article
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin ; New York : Springer International, c1984-
    • الموضوع:
      Asparaginase/*biosynthesis ; Biotechnology/*methods ; Culture Media/*chemistry ; Pectobacterium carotovorum/*enzymology; Antineoplastic Agents/metabolism ; Asparaginase/chemistry ; Asparagine/metabolism ; Glucose/metabolism ; Glutaminase/analysis ; Models, Statistical ; Pectobacterium carotovorum/growth & development
    • نبذة مختصرة :
      Glutaminase-free L-asparaginase is known to be an excellent anticancer agent. In the present study, statistically based experimental designs were applied to maximize the production of glutaminase-free L-asparaginase from Pectobacterium carotovorum MTCC 1428. Nine components of the medium were examined for their significance on the production of L-asparaginase using the Plackett-Burman experimental design. The medium components, viz., glucose, L-asparagine, KH2PO4, and MgSO(4).7H2O, were screened based on their high confidence levels (P<0.04). The optimum levels of glucose, L-asparagine, KH2PO4, and MgSO(4).7H2O were found to be 2.076, 5.202, 1.773, and 0.373 g L(-1), respectively, using the central composite experimental design. The maximum specific activity of L-asparaginase in the optimized medium was 27.88 U mg(-1) of protein, resulting in an overall 8.3-fold increase in the production compared to the unoptimized medium.
    • الرقم المعرف:
      0 (Antineoplastic Agents)
      0 (Culture Media)
      7006-34-0 (Asparagine)
      EC 3.5.1.1 (Asparaginase)
      EC 3.5.1.2 (Glutaminase)
      IY9XDZ35W2 (Glucose)
    • الموضوع:
      Date Created: 20090409 Date Completed: 20091027 Latest Revision: 20191210
    • الموضوع:
      20231215
    • الرقم المعرف:
      10.1007/s00253-009-1973-0
    • الرقم المعرف:
      19352649