Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Selectable marker genes and unintended changes to the plant transcriptome.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • المؤلفون: Miki B;Miki B; Abdeen A; Manabe Y; MacDonald P
  • المصدر:
    Plant biotechnology journal [Plant Biotechnol J] 2009 Apr; Vol. 7 (3), pp. 211-8. Date of Electronic Publication: 2009 Feb 24.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't; Review
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists Country of Publication: England NLM ID: 101201889 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1467-7652 (Electronic) Linking ISSN: 14677644 NLM ISO Abbreviation: Plant Biotechnol J Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2014- : Oxford Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists
      Original Publication: [Oxford] : Blackwell Pub., c2003-
    • الموضوع:
      Gene Expression Profiling* ; Transgenes*; Plants, Genetically Modified/*genetics; Gene Expression Regulation, Plant ; Genetic Markers ; Genome, Plant ; Mutagenesis, Insertional
    • نبذة مختصرة :
      The intended effect of a selectable marker gene is to confer a novel trait that allows for the selection and recovery of transgenic plants. Unintended effects may also occur as a result of interactions between the selectable marker gene or its regulatory elements and genetic elements at the site of insertion. These are called position effects. Other unintended effects may occur if the selectable marker gene has a range of pleiotropic effects related to the functional and regulatory domains within the coding region or the regulatory elements used to drive expression. Both pleiotropic and position effects may generate unpredictable events depending on the process used for transgenesis and the state of knowledge associated with the selectable marker gene. Although some selectable marker genes, such as the neomycin phosphotransferase type II gene (nptII), have no pleiotropic effects on the transcriptomes of transgenic plants, others, such as the bialaphos resistance gene (bar), have pleiotropic effects. These must be clearly understood and accounted for when evaluating the expression patterns conferred by other co-transforming transgenes under study. The number and kinds of selectable marker genes are large. A detailed understanding of their unintended effects is needed to develop transgenic strategies that will minimize or eliminate unintended and unpredictable changes to plants with newly inserted genes.
    • Number of References:
      53
    • الرقم المعرف:
      0 (Genetic Markers)
    • الموضوع:
      Date Created: 20090306 Date Completed: 20090326 Latest Revision: 20220410
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1111/j.1467-7652.2009.00400.x
    • الرقم المعرف:
      19261135