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Characters of cysteine endopeptidases in wheat endosperm during seed germination and subsequent seedling growth.

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  • المؤلفون: Shi C;Shi C; Xu LL
  • المصدر:
    Journal of integrative plant biology [J Integr Plant Biol] 2009 Jan; Vol. 51 (1), pp. 52-7.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Wiley-Blackwell Pub Country of Publication: China (Republic : 1949- ) NLM ID: 101250502 Publication Model: Print Cited Medium: Internet ISSN: 1744-7909 (Electronic) Linking ISSN: 16729072 NLM ISO Abbreviation: J Integr Plant Biol Subsets: MEDLINE
    • بيانات النشر:
      Publication: [China] : Wiley-Blackwell Pub
      Original Publication: [Carlton South, Victoria] : Blackwell Pub., 2005-
    • الموضوع:
      Cysteine Endopeptidases/*metabolism ; Germination/*physiology ; Seedlings/*enzymology ; Seedlings/*growth & development ; Seeds/*enzymology ; Triticum/*embryology ; Triticum/*enzymology; Enzyme Stability/drug effects ; Germination/drug effects ; Hydrogen-Ion Concentration/drug effects ; Protease Inhibitors/pharmacology ; Seed Storage Proteins/metabolism ; Seedlings/drug effects ; Seeds/drug effects ; Substrate Specificity/drug effects ; Temperature ; Triticum/drug effects
    • نبذة مختصرة :
      The endopeptidases (EPs) in wheat endosperm during seed germination and subsequent seedling growth were characterized by gradient-polyacrylamide gel electrophoresis with gelatin copolymerized into the gel. Four cysteine EPs (EP1, EP2, EP3 and EP4) were detected in wheat endosperm during the 7 d growth after seed imbibition. The results also showed that the activities of all of these EPs increased continuously, and EP2 first appeared and had the highest proteolytic activity among the four EPs in this experimental process. The optimum pH and temperature of all four EPs were 4.0 and 40.0 degrees C. All EPs were completely inhibited by 25 micromol/L E-64 and had no good thermal stabilities, especially EP1. In addition, these EPs had different substrate specificities to albumins, globulins, gliadins and glutenins; the main storage proteins of mature wheat endosperm. Among them, EP2 had the highest proteolytic activities on globulins, gliadins and glutenins, and might be the most important and specific EP with potential to be tightly correlated with seedling development.
    • الرقم المعرف:
      0 (Protease Inhibitors)
      0 (Seed Storage Proteins)
      EC 3.4.22.- (Cysteine Endopeptidases)
    • الموضوع:
      Date Created: 20090127 Date Completed: 20090306 Latest Revision: 20151119
    • الموضوع:
      20250114
    • الرقم المعرف:
      10.1111/j.1744-7909.2008.00778.x
    • الرقم المعرف:
      19166494