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Over-expression of nm23-H1 in HeLa cells provides cells with higher resistance to oxidative stress possibly due to raising intracellular p53 and GPX1.

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  • المؤلفون: An R;An R; Chu YL; Tian C; Dai XX; Chen JH; Shi Q; Han J; Dong XP
  • المصدر:
    Acta pharmacologica Sinica [Acta Pharmacol Sin] 2008 Dec; Vol. 29 (12), pp. 1451-8.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Nature Publishing Group Country of Publication: United States NLM ID: 100956087 Publication Model: Print Cited Medium: Internet ISSN: 1745-7254 (Electronic) Linking ISSN: 16714083 NLM ISO Abbreviation: Acta Pharmacol Sin Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2009- : New York : Nature Publishing Group
      Original Publication: Beijing, China : Science Press, c2000-
    • الموضوع:
      Oxidative Stress*; Glutathione Peroxidase/*metabolism ; NM23 Nucleoside Diphosphate Kinases/*metabolism ; Tumor Suppressor Protein p53/*metabolism; Animals ; Glutathione Peroxidase/genetics ; HeLa Cells ; Humans ; Hydrogen Peroxide/metabolism ; NM23 Nucleoside Diphosphate Kinases/genetics ; Oxidants/metabolism ; Reactive Oxygen Species/metabolism ; Tumor Suppressor Protein p53/genetics ; Glutathione Peroxidase GPX1
    • نبذة مختصرة :
      Aim: To determine whether the antitumor factor nm23 is related with antioxidation.
      Methods: Full-length human nm23-H1 was cloned into a mammalianexpressing vector and transiently introduced into HeLa cells.
      Results: A remarkably low level of reactive oxygen species (ROS) was detected in the cells overexpressing nm23-H1. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays found that the cells transfected with a nm23- H1-expressing plasmid had higher viability and stronger resistance to oxidative stress. Immunoprecipitation tests revealed that endogenous nm23-H1 formed a protein complex with p53. Furthermore, the intracellular levels of p53 and p53- regulated gene GPX1 were obviously increased in the cells overexpressing nm23- H1. The downregulation of p53 in the cells overexpressing nm23-H1 resulted in a higher cellular ROS level and lower cell viability.
      Conclusion: The findings suggest that nm23-H1 may act as a cellular protector against oxidative stress, possibly triggering the p53-related antioxidative pathway.
    • الرقم المعرف:
      0 (NM23 Nucleoside Diphosphate Kinases)
      0 (Oxidants)
      0 (Reactive Oxygen Species)
      0 (Tumor Suppressor Protein p53)
      BBX060AN9V (Hydrogen Peroxide)
      EC 1.11.1.9 (Glutathione Peroxidase)
      EC 2.7.4.6 (NME1 protein, human)
      EC 1.11.1.9 (Glutathione Peroxidase GPX1)
      EC 1.11.1.9 (GPX1 protein, human)
    • الموضوع:
      Date Created: 20081126 Date Completed: 20090417 Latest Revision: 20221207
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1111/j.1745-7254.2008.00902.x
    • الرقم المعرف:
      19026164