Item request has been placed!
×
Item request cannot be made.
×
Processing Request
Characterization of interleukin 8 in woodchucks with chronic hepatitis B and hepatocellular carcinoma.
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
- معلومة اضافية
- المصدر:
Publisher: Nature Pub. Group Country of Publication: England NLM ID: 100953417 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5470 (Electronic) Linking ISSN: 14664879 NLM ISO Abbreviation: Genes Immun Subsets: MEDLINE
- بيانات النشر:
Original Publication: Houndmills, Basingstoke, Hampshire, UK : Nature Pub. Group,
- الموضوع:
Carcinoma, Hepatocellular/
*genetics ;
Hepatitis B/
*genetics ;
Hepatitis B Virus, Woodchuck/
*genetics ;
Interleukin-8/
*genetics ;
Liver Neoplasms, Experimental/
*genetics ;
Marmota/
*genetics;
Amino Acid Motifs ;
Amino Acid Sequence ;
Animals ;
Base Sequence ;
Carcinoma, Hepatocellular/
virology ;
Cell Line ;
Cells, Cultured ;
Conserved Sequence ;
DNA, Complementary/
genetics ;
DNA, Viral/
blood ;
Disease Models, Animal ;
Exons ;
Hepatitis B/
metabolism ;
Hepatitis B/
virology ;
Hepatitis B Virus, Woodchuck/
immunology ;
Hepatitis, Viral, Animal/
genetics ;
Humans ;
Interleukin-8/
metabolism ;
Introns ;
Kidney/
cytology ;
Marmota/
immunology ;
Marmota/
virology ;
Molecular Sequence Data ;
Sequence Homology, Amino Acid ;
Viral Load - نبذة مختصرة :
The eastern woodchuck, Marmota monax, represents a useful animal model to study hepatitis B virus infection in humans. However, immunological studies in this model have been impeded by a lack of basic information about the components of the immune system such as cytokines and chemokines. To clarify the role(s) of interleukin 8 (IL-8) in chronic hepatitis B and hepatocellular carcinoma (HCC) in the woodchuck model, we cloned and characterized the woodchuck IL-8 cDNA and genomic DNA. Sequence analysis revealed that the organization of the wk-IL-8 gene is similar to that of the human IL-8 gene and consists of four exons and three introns. Woodchuck IL-8 protein exhibits the conserved ELRCXC motif of IL-8 and shows 87, 82, 82 and 79% similarity with rabbit, ovine, bovine and human IL-8 proteins, respectively. The biological activity of wk-IL-8 was demonstrated using neutrophil chemotaxis assays. Wk-IL-8 could be readily detected in both tumor and non-tumor tissues with higher expression in the non-tumor tissues in most cases. The results from this study will facilitate the investigation of IL-8 in the immunopathogenesis of hepadnavirus-related diseases by the woodchuck model.
- الرقم المعرف:
0 (DNA, Complementary)
0 (DNA, Viral)
0 (Interleukin-8)
- الموضوع:
Date Created: 20081031 Date Completed: 20090305 Latest Revision: 20090121
- الموضوع:
20231215
- الرقم المعرف:
10.1038/gene.2008.86
- الرقم المعرف:
18971938
No Comments.