Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux.

Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Bacteria/*genetics ; Bacteria/*pathogenicity ; Bacterial Infections/*microbiology ; Bacterial Proteins/*genetics ; Oligonucleotide Array Sequence Analysis/*methods; Bacteria/isolation & purification ; Bacterial Proteins/metabolism ; DNA, Bacterial/genetics ; Drug Resistance, Bacterial ; Humans ; Virulence ; Virulence Factors

Background: Human and animal health is constantly under threat by emerging pathogens that have recently acquired genetic determinants that enhance their survival, transmissibility and virulence. We describe the construction and development of an Active Surveillance of Pathogens (ASP) oligonucleotide microarray, designed to 'actively survey' the genome of a given bacterial pathogen for virulence-associated genes.
Results: The microarray consists of 4958 reporters from 151 bacterial species and include genes for the identification of individual bacterial species as well as mobile genetic elements (transposons, plasmid and phage), virulence genes and antibiotic resistance genes. The ASP microarray was validated with nineteen bacterial pathogens species, including Francisella tularensis, Clostridium difficile, Staphylococcus aureus, Enterococcus faecium and Stenotrophomonas maltophilia. The ASP microarray identified these bacteria, and provided information on potential antibiotic resistance (eg sufamethoxazole resistance and sulfonamide resistance) and virulence determinants including genes likely to be acquired by horizontal gene transfer (e.g. an alpha-haemolysin).
Conclusion: The ASP microarray has potential in the clinic as a diagnostic tool, as a research tool for both known and emerging pathogens, and as an early warning system for pathogenic bacteria that have been recently modified either naturally or deliberately.

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062511 United Kingdom WT_ Wellcome Trust; G0300020 United Kingdom MRC_ Medical Research Council

0 (Bacterial Proteins)
0 (DNA, Bacterial)
0 (Virulence Factors)

Date Created: 20081011 Date Completed: 20090121 Latest Revision: 20220129

20250114

PMC2607285

10.1186/1471-2180-8-177

18844996