Adherence and invasion of Bacteroidales isolated from the human intestinal tract.

Publisher: Elsevier Country of Publication: England NLM ID: 9516420 Publication Model: Print Cited Medium: Internet ISSN: 1469-0691 (Electronic) Linking ISSN: 1198743X NLM ISO Abbreviation: Clin Microbiol Infect Subsets: MEDLINE

Publication: 2015- : London : Elsevier
Original Publication: Paris : Decker Europe, c1995-

Bacterial Adhesion*; Bacteroidetes/*pathogenicity ; Bacteroidetes/*physiology ; Diarrhea/*microbiology ; Gastrointestinal Tract/*microbiology; Animals ; Bacterial Capsules/analysis ; Bacteroidetes/cytology ; Bacteroidetes/isolation & purification ; Cell Line ; Child ; Child, Preschool ; Colony Count, Microbial ; Cytosol/microbiology ; Epithelial Cells/microbiology ; Feces/microbiology ; Fimbriae, Bacterial ; Humans ; Infant ; Microscopy, Electron ; Microscopy, Immunoelectron ; Virulence Factors/analysis

Members of the genera Bacteroides and Parabacteroides are important constituents of both human and animal intestinal microbiota, and are significant facultative pathogens. In this study, the ability of Bacteroides spp. and Parabacteroides distasonis isolated from both diarrhoeal and normal stools (n = 114) to adhere to and invade HEp-2 cells was evaluated. The presence of putative virulence factors such as capsule and fimbriae was also investigated. Adherence to HEp-2 cells was observed in 75.4% of the strains, which displayed non-localized clusters. Invasion was observed in 37.5% and 26% of the strains isolated from diarrhoeal and non-diarrhoeal stools, respectively. All strains displayed a capsule, whereas none of them showed fimbriae-like structures. This is the first report of the ability of Bacteroides spp. and P. distasonis to adhere to and invade cultured HEp-2 epithelial cells.

0 (Virulence Factors)

Date Created: 20081003 Date Completed: 20081106 Latest Revision: 20121115

20240829

10.1111/j.1469-0691.2008.02069.x

18828854