Cloning and characterisation of a maize carotenoid cleavage dioxygenase (ZmCCD1) and its involvement in the biosynthesis of apocarotenoids with various roles in mutualistic and parasitic interactions.

Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0032-0935 (Print) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE

Original Publication: Berlin, New York, Springer-Verlag [etc.]

Carotenoids/*metabolism ; Dioxygenases/*genetics ; Plant Proteins/*genetics ; Zea mays/*genetics; Amino Acid Sequence ; Cloning, Molecular ; Dicarboxylic Acids/metabolism ; Dioxygenases/chemistry ; Dioxygenases/metabolism ; Host-Pathogen Interactions ; Models, Biological ; Molecular Sequence Data ; Molecular Structure ; Mycorrhizae/growth & development ; Mycorrhizae/physiology ; Plant Proteins/chemistry ; Plant Proteins/metabolism ; Plant Roots/genetics ; Plant Roots/metabolism ; Plant Roots/microbiology ; Polyenes/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Striga/growth & development ; Striga/microbiology ; Zea mays/enzymology ; Zea mays/microbiology

Colonisation of maize roots by arbuscular mycorrhizal (AM) fungi leads to the accumulation of apocarotenoids (cyclohexenone and mycorradicin derivatives). Other root apocarotenoids (strigolactones) are involved in signalling during early steps of the AM symbiosis but also in stimulation of germination of parasitic plant seeds. Both apocarotenoid classes are predicted to originate from cleavage of a carotenoid substrate by a carotenoid cleavage dioxygenase (CCD), but the precursors and cleavage enzymes are unknown. A Zea mays CCD (ZmCCD1) was cloned by RT-PCR and characterised by expression in carotenoid accumulating E. coli strains and analysis of cleavage products using GC-MS. ZmCCD1 efficiently cleaves carotenoids at the 9, 10 position and displays 78% amino acid identity to Arabidopsis thaliana CCD1 having similar properties. ZmCCD1 transcript levels were shown to be elevated upon root colonisation by AM fungi. Mycorrhization led to a decrease in seed germination of the parasitic plant Striga hermonthica as examined in a bioassay. ZmCCD1 is proposed to be involved in cyclohexenone and mycorradicin formation in mycorrhizal maize roots but not in strigolactone formation.

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0 (Dicarboxylic Acids)
0 (Plant Proteins)
0 (Polyenes)
160162-46-9 (mycorradicin)
36-88-4 (Carotenoids)
EC 1.13.11.- (Dioxygenases)
EC 1.13.11.- (carotenoid cleavage dioxygenase 1)

Date Created: 20080822 Date Completed: 20090331 Latest Revision: 20211020

20221213

10.1007/s00425-008-0781-6

18716794