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Effects of viral silencing suppressors on tobacco ringspot virus infection in two Nicotiana species.

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Microbiology Society Country of Publication: England NLM ID: 0077340 Publication Model: Print Cited Medium: Print ISSN: 0022-1317 (Print) Linking ISSN: 00221317 NLM ISO Abbreviation: J Gen Virol Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2015- : London : Microbiology Society
      Original Publication: London, Cambridge Univ. Press for the Society for General Microbiology.
    • الموضوع:
      Gene Silencing* ; Genes, Suppressor*; Genes, Viral/*genetics ; Nepovirus/*physiology ; Plant Diseases/*virology ; Nicotiana/*virology ; Viral Proteins/*genetics; Cysteine Endopeptidases/genetics ; Molecular Sequence Data ; Nepovirus/pathogenicity ; Plant Leaves/virology ; Plants, Genetically Modified ; RNA, Small Interfering/metabolism ; Temperature ; Nicotiana/genetics ; Viral Proteins/metabolism ; Virulence ; Virus Replication
    • نبذة مختصرة :
      This study investigated the effects of silencing suppressors derived from six different viruses (P1, P19, P25, HcPro, AC2 and 2b), expressed in transgenic Nicotiana tabacum and Nicotiana benthamiana plants, on the infection pattern of tobacco ringspot virus (TRSV) potato calico strain. In N. benthamiana, this virus produced an initial infection with severe systemic symptoms, but the infection was strongly reduced within a few weeks as the plant recovered from the infection. P25 and HcPro silencing suppressors effectively prevented recovery in this host, allowing continuous accumulation of the viral RNA as well as of the virus-specific small interfering RNAs, in the systemically infected leaves. In the P1-, P19-, AC2- or 2b-expressing transgenic N. benthamiana, the recovery was not complete. Susceptibility of N. tabacum to this virus was temperature sensitive. At lower temperatures, up to 25 degrees C, the plants became systemically infected, but at higher temperatures, the infections were limited to the inoculated leaves. In these preventative conditions, all silencing suppressor transgenes (except P25, which was expressed at very low levels) allowed the establishment of systemic infections. Very strong and consistent systemic infections were observed in HcPro- and AC2-expressing plants.
    • Molecular Sequence:
      GENBANK EU281548
    • الرقم المعرف:
      0 (RNA, Small Interfering)
      0 (Viral Proteins)
      EC 3.4.22.- (Cysteine Endopeptidases)
      EC 3.4.22.- (HC-Pro protein, potyvirus)
    • الموضوع:
      Date Created: 20080514 Date Completed: 20080701 Latest Revision: 20231213
    • الموضوع:
      20240628
    • الرقم المعرف:
      10.1099/vir.0.83621-0
    • الرقم المعرف:
      18474567