Physicochemical and Immunological Properties of RiVax Loaded in PLGA Nanoparticles.

Introduction: Ricin is a glycoprotein poison found in the castor plant, which consists of two chains A (RTA) and B (RTB). There are two protein vaccine candidates based on chain A including RiVax and RVEc, against poisoning caused by this toxin. Although various studies have been conducted on the immunogenicity of RiVax alone and with the help of adjuvant, modern controlled release systems such as nanoparticles have not been used to improve the immunogenicity of this protein. The aim of this study is to manufacture polylactic-co-glycolic acid (PLGA) nanoparticles containing RiVax with a slow release rate and to evaluate the physicochemical parameters and immunogenic potential of this system compared to protein alone. Methods: First, after purifying RiVax, the water-oil-water emulsion method was used to load RiVax into poly lactic acid-co-glycolic acid (PLGA) nanoparticles. After investigating the physicochemical properties of nanoparticles containing RiVax, they were administered to different groups of mice. Results: According to the above results, it was determined that despite the various researches and positive results related to the nature of immunogenicity enhancement by PLGA nanoparticles, one of the important factors in the enhancement of immunogenicity of these nanoparticles is the ratio of lactic acid to glycolic acid that constitutes PLGA, which has a significant effect on the antigen release rate or on the immune response. [ABSTRACT FROM AUTHOR]

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