The Effect of Soil Tillage Systems on the Soil Microbial and Enzymatic Properties Under Soybean (Glycine max L. Merrill) Cultivation—Implications for Sustainable Soil Management.

Reducing soil tillage with the application of catch-crop green mass as a mulch is a conservation practice that is used in agriculture to improve soil ecosystem functioning. Such a cultivation method enhances soil organic matter quantity and quality through the improvement of soil biological activity and nutrient availability, while reducing soil disturbance. Therefore, a three-year field experiment was conducted in the years 2017–2019 to evaluate the effect of three tillage methods (TMs) (conventional, CT; reduced, RT; and strip tillage, ST) on soil microbial and specific enzyme properties (microbial C and N content, the activity of dehydrogenases—DHA, the rate of fluorescein sodium salt hydrolysis—FDAH, CMC-cellulase—Cel and β-glucosidase—Glu) and certain basic soil properties. The study was performed in a field; it was a one-factor experiment that was carried out in a randomized block design. The soil samples were collected from the upper soil layer five times a year: in April (before the sowing of soybean), May, June, August and September (before soybean harvesting). The tillage methods or sampling dates used had no significant effect on the organic carbon and total nitrogen levels. Most of the C-related properties (the content of microbial biomass and the C-cycling enzymatic activity such as Cel and Glu) and microbial activity bioindicators (DHA activity, FDAH rate) revealed significant seasonal changes, whereby each variable was affected in a different order (e.g., the Cel activity was significantly higher in April and September than in other months—22%, while the DHA activity was significantly higher in June and August compared to other months—18%). RT significantly increased the enzymatic activity as compared to CT and ST, and the difference was between 8 and 33% (with a mean of 18%). The exception was the β-glucosidase activity as determined in 2019, which was significantly higher in the case of CT (1.02 mg pNP kg−1 h−1) and ST than in RT (0.705 mg pNP kg−1 h−1). However, the explanation for such phenomenon could not possibly be based on the available data. Our results suggested that the response of the enzyme activities toward the same factor may be due to the inherent variability in enzyme response associated with the spatial variability in soil properties as well as the properties of the enzyme itself and changes in the periodic occurrence of its substrates in the soil. Generally, the reduced tillage combined with plant residues return could be recommended for enhancing soil health and quality by improving its microbial and enzymatic features. The findings above suggest that a reduced tillage system is an important component of soil management in sustainable agriculture. [ABSTRACT FROM AUTHOR]