Laminin γ, High Mobility Group Box I (HMGB1), and Collagen Type-1 for Detection of Enterococcus Faecalis Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs).

The inflamed pulp can develop into pulp necrosis and requires endodontic treatment. The failure of endodontic treatment is mainly caused by Enterococcus faecalis infection. The mechanism is mediated by the ability of E. faecalis, as a gram-positive bacterium, to form a biofilm covering the cell membrane, which is known as microbial surface recognizing adhesive matrix molecules (MSCRAMMs). The aim of this study was to identify MSCRAMMs through the expression of lamininγ, High Mobility Group Box I (HMGB1), and type-1 collagen. All groups were prepared for molar cavitation using a round bur and injected with E. faecalis bacteria with a concentration of 100%. They were observed for 24 hours, 72 hours, and 120 hours after injection. The K group was the control group containing rats that were not received a bacterial injection at 24 hours, 72 hours, and 120 hours. After that, the tooth was decalcified using 10% EDTA at 37 °C, which took up to two months. Macrophages were observed using a light binocular microscope. Significant results (p<0.05) were found in laminin γ and HMGB1 between the control with 24 hours groups, also insignificant results (p>0.05) were found between 24 with 72 hours group and 72 with 120 hours group. All groups within the collagen type-1 group were not significant. Both laminin γ and HMGB1 are good parameters to predict Enterococcus faecalis growth phase and biofilms production with the log phase happening before 24 hours and the stationary phase happening after 24 hours. [ABSTRACT FROM AUTHOR]

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