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Optimization and Stability Assessment of Monochamus alternatus Antimicrobial Peptide MaltAtt-1 in Komagataella phaffii GS115 for the Control of Pine Wood Nematode.
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- المؤلفون: Jiang, Di1,2 (AUTHOR); Xu, Xuhuizi1,2 (AUTHOR); Wang, Zeguang1,2 (AUTHOR); Yu, Chao1 (AUTHOR); Wang, Zeqing1 (AUTHOR); Xu, Yuda1,2 (AUTHOR); Chu, Xu1,2 (AUTHOR); Li, Ming1 (AUTHOR); Zhang, Feiping1,2 (AUTHOR); Hu, Xia1,2 (AUTHOR)
- المصدر:
International Journal of Molecular Sciences. Aug2024, Vol. 25 Issue 16, p8555. 13p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
MaltAtt-1 is an antimicrobial peptide isolated from Monochamus alternatus with nematocidal activity against pine wood nematode. In this study, a eukaryotic expression system based on Komagataella phaffii GS115 was established, and its secretory expression of MaltAtt-1 was realized. The basic properties and secondary and tertiary structures of the antimicrobial peptide MaltAtt-1 were identified by bioinformatics analysis. MaltAtt-1 is a hydrophilic stable protein, mainly composed of an α-helix (Hh), β-folds (Ee), and irregular curls (Cc). The optimal fermentation conditions for MaltAtt-1 were determined by a single-factor test and the Box–Behnken response surface method, including an induction time of 72 h, induction temperature of 30 °C, culture medium of pH 7.6, methanol volume fraction of 2.0%, and an initial glycerol concentration of 1%. The stability of MaltAtt-1 indicated its resistant to UV irradiation and repeated freezing and thawing, but the antibacterial activity decreased significantly under the influence of high temperature and a strong acid and base, and it decreased significantly to 1.1 cm and 0.83 cm at pH 2.0 and pH 10.0, respectively. The corrected mortality of B. xylophilus achieved 71.94% in 3 h at a concentration of 300 mg·L−1 MaltAtt-1 exposure. The results provide a theoretical basis for the antimicrobial peptide MaltAtt-1 to become a new green and efficient nematicide. [ABSTRACT FROM AUTHOR]
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