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Evaluation of bEnd5 cell line as an in vitro model for the blood-brain barrier under normal and hypoxic/aglycemic conditions.

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  • المؤلفون: Yang T;Yang T; Roder KE; Abbruscato TJ
  • المصدر:
    Journal of pharmaceutical sciences [J Pharm Sci] 2007 Dec; Vol. 96 (12), pp. 3196-213.
  • نوع النشر :
    Comparative Study; Evaluation Study; Journal Article; Research Support, N.I.H., Extramural
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Elsevier Country of Publication: United States NLM ID: 2985195R Publication Model: Print Cited Medium: Print ISSN: 0022-3549 (Print) Linking ISSN: 00223549 NLM ISO Abbreviation: J Pharm Sci Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2016- : New York, NY : Elsevier
      Original Publication: Easton, Pa., American Pharmaceutical Assn.
    • الموضوع:
    • نبذة مختصرة :
      The purpose of the study was to assess the suitability of the mouse endothelial cell line bEnd5 as a blood-brain barrier (BBB) model under normal or pathologic (stroke) conditions. In comparison to the well-established bovine brain endothelial cell (BBMEC) model, cultured bEnd5 monolayers reached a maximal transendothelial electrical resistance (TEER) of 121 Omega cm(2) on day 7, and possessed oval and spindle shape morphology. Structurally, confluent monolayers of bEnd5 cells and BBMECs exhibit peripheral band staining of the tight junction protein ZO-1 and occludin. Both bEnd5 and BBMECs express important tight junctional proteins, ZO-1, occludin and claudin-1, as well as the transporters P-glycoprotein (P-gp), NKCC, GLUT1, and most PKC isoforms. Marker permeability experiments suggest that bEnd5 cells form a tight barrier that compares to well-established in vitro BBB models, such as the BBMEC. After short durations of hypoxia/aglycemia (H/A), hyperpermeability was seen in the bEnd5 endothelial monolayer compared to later time periods for BBMECs, suggesting that bEnd5 cells are more sensitive to hypoxia/algycemia treatment than BBMECs. Taken together, bEnd5 cell culture model may provide a useful in vitro model of the BBB for drug delivery studies and modeling pathological states such as oxygen glucose deprivation associated with stroke.
      ((c) 2007 Wiley-Liss, Inc.)
    • Grant Information:
      NS 046526 United States NS NINDS NIH HHS
    • الرقم المعرف:
      0 (Cadherins)
      0 (Claudin-1)
      0 (Cldn1 protein, mouse)
      0 (Isoenzymes)
      0 (Membrane Proteins)
      0 (Membrane Transport Proteins)
      0 (Occludin)
      0 (Ocln protein, mouse)
      0 (Phosphoproteins)
      0 (Tjp1 protein, mouse)
      0 (Zonula Occludens-1 Protein)
      57-50-1 (Sucrose)
      9Y8NXQ24VQ (Propranolol)
      EC 2.7.11.13 (Protein Kinase C)
      IY9XDZ35W2 (Glucose)
    • الموضوع:
      Date Created: 20070911 Date Completed: 20080214 Latest Revision: 20191210
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1002/jps.21002
    • الرقم المعرف:
      17828743