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Oligomeric compounds formed from 2,5-xylidine (2,5-dimethylaniline) are potent enhancers of laccase production in Trametes versicolor ATCC 32745.
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- المؤلفون: Kollmann, Albert; Boyer, François-Didier; Ducrot, Paul-Henri; Kerhoas, Lucien; Jolivalt, Claude; Touton, Isabelle; Einhorn, Jacques; Mougin, Christian
- المصدر:
Applied Microbiology & Biotechnology; Aug2005, Vol. 68 Issue 2, p251-258, 8p, 1 Diagram, 1 Chart, 2 Graphs
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
Numerous chemicals, including the xenobiotic 2,5-xylidine, are known to induce laccase production in fungi. The present study was conducted to determine whether the metabolites formed from 2,5-xylidine by fungi could enhance laccase activity. We used purified laccases to transform the chemical and then we separated the metabolites, identified their chemical structure and assayed their effect on enzyme activity in liquid cultures of Trametes. versicolor. We identified 13 oligomers formed from 2,5-xylidine. (4 E)-4-(2,5-dimethylphenylimino)-2,5-dimethylcyclohexa-2,5-dienone at 1.25×10−5 M was an efficient inducer, resulting in a nine-fold increase of laccase activity after 3 days of culture. Easily synthesized in one step (67% yield), this compound could be used in fungal bioreactors to obtain a great amount of laccases for biochemical or biotechnological purposes, with a low amount of inducer. [ABSTRACT FROM AUTHOR]
- نبذة مختصرة :
Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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