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Structural Characterization and In Vitro Anti-Inflammatory Activity of Polysaccharides Isolated from the Fruits of Rosa laevigata.
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- المؤلفون: Peng, Song1,2 (AUTHOR) ; Gu, Pengfei3 (AUTHOR) ; Mao, Ningning1,2 (AUTHOR) ; Yu, Lin1,2 (AUTHOR) ; Zhu, Tianyu1,2 (AUTHOR) ; He, Jin1,2 (AUTHOR) ; Yang, Yang1,2 (AUTHOR) ; Liu, Zhenguang1,2 (AUTHOR) ; Wang, Deyun1,2 (AUTHOR)
- المصدر:
International Journal of Molecular Sciences. Feb2024, Vol. 25 Issue 4, p2133. 15p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
RLPa-2 (Mw 15.6 kDa) is a polysaccharide isolated from Rosa laevigata Michx. It consists of arabinose (Ara), galactose (Gal), rhamnose (Rha), glucose (Glc), xylose (Xyl), and galacturonic acid (Gal-UA) with a molar ratio of 1.00:0.91:0.39:0.34:0.25:0.20. Structural characterization was performed by methylation and NMR analysis, which indicated that RLPa-2 might comprise →6)-α-D-Galp-(1→, →4)-α-D-GalpA-(1→, α-L-Araf-(1→, →2,4)-α-D-Glcp-(1→, β-D-Xylp, and α-L-Rhap. In addition, the bioactivity of RLPa-2 was assessed through an in vitro macrophage polarization assay. Compared to positive controls, there was a significant decrease in the expression of M1 macrophage markers (CD80, CD86) and p-STAT3/STAT3 protein. Additionally, there was a down-regulation in the production of pro-inflammatory mediators (NO, IL-6, TNF-α), indicating that M1 macrophage polarization induced with lipopolysaccharide (LPS) and interferon-γ (IFN-γ) stimulation could be inhibited by RLPa-2. These findings demonstrate that the RLPa-2 might be considered as a potential anti-inflammatory drug to reduce inflammation. [ABSTRACT FROM AUTHOR]
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