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بررسی اثر حفاظت فراسرد بر شاخصهای رشد و ترکیب بیوشیمیایی Chaetoceros calcitrans ریزجلبک

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  • معلومة اضافية
    • Alternate Title:
      Cryopreservation of the microalgae Chaetoceros calcitrans and its effects on growth and biochemical composition.
    • نبذة مختصرة :
      In this study, in order to evaluate the possibility of cryopreservation of C. calcitrans, 4 cryoprotectant agents (dimethyl sulfoxide, methanol, glycerol and ethylene glycol), each with different concentrations (2.5, 5, 10, 15, 20, 25% (v/v)), were taken as experimental treatments. Then, microalgae samples were placed at -196°C for 30 days. After that, in order to determine the best treatments, re-cultivation of microalgae was carried out and growth performance, biochemical composition and fatty acid profile were measured. After thawing, regrowth of microalgae cells was observed in 15 out of 24 treatments. The highest final cell density (4.13x106 cells/ml) was observed in DMSO15. After thawing, in the treatments in which the cells were reproduced again, the viability ranged between 8.26 and 28.19 percent. Maximum viability index were recorded in DMSO15, DMSO10 and ME15, respectively. Biochemical composition analysis showed that there was a significant increase on the lipid content in the experimental treatments compared to the control group (p<0.05). No significant difference was observed on the fatty acid profile(p>0.05). The results showed that the cryopreservation of C. calcitrans can be successful only if we use DMSO (5-15%) or ME (10-15%) as a cryoprotectant, and the best results are obtained when using DMSO at the level of 15%. [ABSTRACT FROM AUTHOR]
    • نبذة مختصرة :
      Copyright of Journal of Fisheries is the property of University of Tehran and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)