Hoc protein regulates the biological effects of T4 phage in mammals.

Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0302-8933 (Print) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE

Original Publication: Berlin, New York, Springer-Verlag.

Gene Expression Regulation, Viral* ; Mutation*; Bacteriophage T4/*physiology ; Capsid Proteins/*metabolism ; Melanoma, Experimental/*therapy ; Viral Proteins/*metabolism; Animals ; Antineoplastic Agents/pharmacology ; Bacteriophage T4/classification ; Bacteriophage T4/genetics ; Bacteriophage T4/ultrastructure ; Capsid Proteins/genetics ; Female ; Male ; Melanoma, Experimental/secondary ; Melanoma, Experimental/virology ; Mice ; Mice, Inbred C57BL ; Organ Specificity ; Viral Proteins/genetics

We previously investigated the biological, non-antibacterial effects of bacteriophage T4 in mammals (binding to cancer cells in vitro and attenuating tumour growth and metastases in vivo); we selected the phage mutant HAP1 that was significantly more effective than T4. In this study we describe a non-sense mutation in the hoc gene that differentiates bacteriophage HAP1 and its parental strain T4. We found no substantial effects of the mutation on the mutant morphology, and its effects on electrophoretic mobility and hydrodynamic size were moderate. Only the high ionic strength of the environment resulted in a size difference of about 10 nm between T4 and HAP1. We compared the antimetastatic activity of the T2 phage, which does not express protein Hoc, with those of T4 and HAP1 (B16 melanoma lung colonies). We found that HAP1 and T2 decreased metastases with equal effect, more strongly than did T4. We also investigated concentrations of T4 and HAP1 in the murine blood, tumour (B16), spleen, liver, or muscle. We found that HAP1 was rapidly cleared from the organism, most probably by the liver. Although HAP1 was previously defined to bind cancer cells more effectively (than T4), its rapid elimination precluded its higher concentration in tumours.

0 (Antineoplastic Agents)
0 (Capsid Proteins)
0 (HOC protein, T4 phage)
0 (Viral Proteins)

Date Created: 20070213 Date Completed: 20071009 Latest Revision: 20220330

20231215

10.1007/s00203-007-0216-y

17294171