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Expression of granzyme A in human polymorphonuclear neutrophils.

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  • معلومة اضافية
    • المصدر:
      Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 0374672 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0019-2805 (Print) Linking ISSN: 00192805 NLM ISO Abbreviation: Immunology Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Oxford : Blackwell Scientific Publications
    • الموضوع:
      Granzymes/*blood ; Neutrophils/*enzymology; Cell Fractionation ; Cells, Cultured ; Gene Expression Regulation, Enzymologic ; Granzymes/genetics ; HL-60 Cells ; Humans ; Phagocytosis ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Subcellular Fractions/enzymology ; Up-Regulation
    • نبذة مختصرة :
      Polymorphonuclear neutrophils (PMNs) are the first line of defence against invading pathogens. They contain a multitude of antimicrobial and potentially cytotoxic substances packed in granules and secretory vesicles. Here, we show that granzyme A (GzmA) is constitutively expressed in human PMNs, but not in the promyelocytic cell line HL-60, by performing flow cytometry, western blot, enzyme-linked immunosorbent assay and quantitative polymerase chain reaction. To further track the intracellular localization of GzmA, we performed subcellular fractionation and found GzmA to be present in peroxidase-negative granules. Finally, stimulation with opsonized Escherichia coli or the bioincompatible haemodialysis membrane cuprophane led to up-regulation of GzmA expression at the transcriptional level as well as at the translational level. In conclusion, we provide clear evidence that GzmA is constitutively expressed in human PMNs and can be up-regulated upon stimulation. These findings may also indicate a role for GzmA in PMNs in defence against invading pathogens.
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    • الرقم المعرف:
      EC 3.4.21.- (Granzymes)
    • الموضوع:
      Date Created: 20070125 Date Completed: 20070618 Latest Revision: 20181113
    • الموضوع:
      20221213
    • الرقم المعرف:
      PMC2265930
    • الرقم المعرف:
      10.1111/j.1365-2567.2006.02551.x
    • الرقم المعرف:
      17244155