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Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic Progenitors.
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- المؤلفون: Dalle Carbonare, Luca1 (AUTHOR) ; Minoia, Arianna1 (AUTHOR) ; Braggio, Michele1 (AUTHOR) ; Bertacco, Jessica2 (AUTHOR) ; Piritore, Francesca Cristiana2 (AUTHOR) ; Zouari, Sharazed1 (AUTHOR) ; Vareschi, Anna1 (AUTHOR) ; Elia, Rossella1 (AUTHOR) ; Vedovi, Ermes3 (AUTHOR) ; Scumà, Cristina3 (AUTHOR) ; Carlucci, Matilde4 (AUTHOR) ; Bhandary, Lekhana5 (AUTHOR) ; Mottes, Monica2 (AUTHOR) ; Romanelli, Maria Grazia2 (AUTHOR) ; Valenti, Maria Teresa2 (AUTHOR)
- المصدر:
International Journal of Molecular Sciences. Sep2023, Vol. 24 Issue 17, p13163. 18p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
The finding of molecules associated with aging is important for the prevention of chronic degenerative diseases and for longevity strategies. MicroRNAs (miRNAs) are post-transcriptional regulators involved in many biological processes and miR-146b-5p has been shown to be involved in different degenerative diseases. However, miR-146b-5p modulation has not been evaluated in mesenchymal stem cells (MSCs) commitment or during aging. Therefore, the modulation of miR-146b-5p in the commitment and differentiation of mesenchymal cells as well as during maturation and aging in zebrafish model were analyzed. In addition, circulating miR-146b-5p was evaluated in human subjects at different age ranges. Thus, the role of physical activity in the modulation of miR-146b-5p was also investigated. To achieve these aims, RT (real-time)-PCR, Western blot, cell transfections, and three-dimensional (3D) culture techniques were applied. Our findings show that miR-146b-5p expression drives MSCs to adipogenic differentiation and increases during zebrafish maturation and aging. In addition, miR-146b-5p expression is higher in females compared to males and it is associated with the aging in humans. Interestingly, we also observed that the physical activity of walking downregulates circulating miR-146b-5p levels in human females and increases the number of chondroprogenitors. In conclusion, miR-146b-5p can be considered an age-related marker and can represent a useful marker for identifying strategies, such as physical activity, aimed at counteracting the degenerative processes of aging. [ABSTRACT FROM AUTHOR]
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