Synthesis of overlapping fibrin citrullinated peptides and their use for diagnosing rheumatoid arthritis.

Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101262549 Publication Model: Print Cited Medium: Print ISSN: 1747-0277 (Print) Linking ISSN: 17470277 NLM ISO Abbreviation: Chem Biol Drug Des Subsets: MEDLINE

Original Publication: Oxford : Wiley-Blackwell, 2006-

Arthritis, Rheumatoid/*diagnosis ; Citrulline/*analogs & derivatives ; Fibrin/*chemical synthesis ; Peptide Fragments/*chemical synthesis ; Peptides, Cyclic/*chemical synthesis; Amino Acid Sequence ; Arthritis, Rheumatoid/blood ; Arthritis, Rheumatoid/immunology ; Chromatography, High Pressure Liquid/methods ; Citrulline/chemistry ; Enzyme-Linked Immunosorbent Assay/methods ; Epitope Mapping/methods ; Fibrin/chemistry ; Fibrin/immunology ; Filaggrin Proteins ; Humans ; Immunodominant Epitopes/immunology ; Intermediate Filament Proteins/chemistry ; Intermediate Filament Proteins/immunology ; Molecular Sequence Data ; Peptide Fragments/chemistry ; Peptide Fragments/immunology ; Peptides, Cyclic/chemistry ; Peptides, Cyclic/immunology ; Sensitivity and Specificity ; Sequence Analysis, Protein/methods

With the aim of developing a new enzyme-linked immunosorbent assay test to detect autoantibodies in the sera of rheumatoid arthritis patients with a high sensitivity and specificity using synthetic citrullinated peptides of fibrin (which is abundant in rheumatoid synovium) as antigenic substract, peptides belonging to alpha- and beta-fibrin chains were selected by computer-aided prediction of antigenicity and epitope mapping and synthesized in solid phase. We analysed by enzyme-linked immunosorbent assay 133 sera from patients with well-characterized rheumatic diseases, including 67 patients with rheumatoid arthritis. The results of the immunoassays reported highlight the usefulness of fibrin-related peptides in rheumatoid arthritis diagnosis and, especially, the ability and specificity of the [Cit(621,627,630)]alpha-fibrin(617-631) (alpha fib617) peptide sequence to recognize the autoantibodies that are present in rheumatoid arthritis patients.

0 (Filaggrin Proteins)
0 (Immunodominant Epitopes)
0 (Intermediate Filament Proteins)
0 (Peptide Fragments)
0 (Peptides, Cyclic)
0 (cyclic citrullinated peptide)
29VT07BGDA (Citrulline)
9001-31-4 (Fibrin)

Date Created: 20061116 Date Completed: 20070313 Latest Revision: 20211203

20221213

10.1111/j.1747-0285.2006.00438.x

17105483