Glucomannan enhanced the macrophage activity in exposure to methicillin-resistant Staphylococcus aureus (MRSA): in-vitro study.

Background and Objectives: The increasing number of methicillin-resistant Staphylococcus aureus persuade the need for preventive measures. Glucomannan is a polysaccharide choice for developing immunological strategies. This study aimed to investigate changes in gene expression and phagocytic activity of macrophage cells in the presence of glucomannan. Materials and Methods: The effect of different concentrations of glucomannan (25, 50, and 100 µg/mL) on the phagocytic activity of macrophage cells was measured using the colony count method. The expression of Tumor Necrosis Factor-alpha (TNF-a) and Inducible Nitric Oxide Synthase (iNOS) genes was evaluated by Real-Time PCR. Results: The concentrations of glucomannan significantly reduced the bacterial Colony-Forming Unit (CFU) and increased the phagocytic activity of macrophage cells. The maximum effect of glucomannan on iNOS and TNF-γ genes expression was 100 µg/mL. Conclusion: Glucomannan should be considered an adjuvant that stimulates the immune system. It may increase the expression of TNF-a and iNOS genes and the phagocytic activity of macrophage cells against methicillin-resistant Staphylococcus aureus. [ABSTRACT FROM AUTHOR]

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