SNP identification, linkage and radiation hybrid mapping of the porcine lamin A/C (LMNA) gene to chromosome 4q.

Publisher: Blackwell Country of Publication: Germany NLM ID: 100955807 Publication Model: Print Cited Medium: Print ISSN: 0931-2668 (Print) Linking ISSN: 09312668 NLM ISO Abbreviation: J Anim Breed Genet Subsets: MEDLINE

Publication: 1994- : Berlin : Blackwell
Original Publication: Hamburg : P. Parey, c1985-

Chromosomes, Mammalian/*genetics ; Genetic Linkage/*genetics ; Lamin Type A/*genetics ; Polymorphism, Single Nucleotide/*genetics ; Radiation Hybrid Mapping/*veterinary ; Swine/*genetics; Alleles ; Animals

The lamins are components of nuclear lamina and they have a profound influence on nuclear structure and functions. They are encoded by three genes, LMNA, LMNB1 and LMNB2. A genomic fragment of the porcine LMNA gene (822 bp; from exons 7 to 9) was amplified by polymerase chain reaction and comparatively sequenced. Four single nucleotide polymorphisms (SNPs) were identified in intronic sequences: G162A, G208A, T367G and C618T. The SNPs are within the restriction sites for enzymes Bsh1236I, HpaII, AluI and Bsh1236I respectively. Allele frequencies at SNPs G208A, T367G and C618T were determined by using eight pig breeds. Linkage analysis in the Hohenheim Meishan x Piétrain family placed the LMNA gene in the chromosome 4q linkage group, between MEF2D and GBA (MEF2D - 3.0 cM - LMNA - 0.2 cM - GBA). In radiation hybrid mapping LMNA was most significantly linked to SW270 on chromosome 4 (39 cR; LOD = 7.86). The LMNA gene is located in the quantitative trait loci region for some carcass traits on chromosome 4q.

0 (Lamin Type A)

Date Created: 20060803 Date Completed: 20070119 Latest Revision: 20101118

20250114

10.1111/j.1439-0388.2006.00591.x

16882095