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RNA interference machinery influences the nuclear organization of a chromatin insulator.

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  • المؤلفون: Lei EP;Lei EP; Corces VG
  • المصدر:
    Nature genetics [Nat Genet] 2006 Aug; Vol. 38 (8), pp. 936-41. Date of Electronic Publication: 2006 Jul 23.
  • نوع النشر :
    Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Nature Pub. Co Country of Publication: United States NLM ID: 9216904 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1061-4036 (Print) Linking ISSN: 10614036 NLM ISO Abbreviation: Nat Genet Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: New York, NY : Nature Pub. Co., c1992-
    • الموضوع:
      Insulator Elements* ; RNA Interference*; Chromatin/*genetics ; Drosophila melanogaster/*genetics; Animals ; Cell Nucleus/genetics ; Female ; Genes, Insect ; Models, Genetic ; Mutation ; Recombinant Proteins/genetics ; Transcription Factors/genetics
    • نبذة مختصرة :
      RNA interference (RNAi) is a conserved silencing mechanism that can act through alteration of chromatin structure. Chromatin insulators promote higher-order nuclear organization, thereby establishing DNA domains subject to distinct transcriptional controls. We present evidence for a functional relationship between RNAi and the gypsy insulator of D. melanogaster. Insulator activity is decreased when Argonaute genes required for RNAi are mutated, and insulator function is improved when the levels of the Rm62 helicase, involved in double-stranded RNA (dsRNA)-mediated silencing and heterochromatin formation, are reduced. Rm62 interacts physically with the DNA-binding insulator protein CP190 in an RNA-dependent manner. Finally, reduction of Rm62 levels results in marked nuclear reorganization of a compromised insulator. These results suggest that the RNAi machinery acts as a modulator of nuclear architecture capable of effecting global changes in gene expression.
    • الرقم المعرف:
      0 (Chromatin)
      0 (Recombinant Proteins)
      0 (Transcription Factors)
      0 (mdg4 protein (gypsy))
    • الموضوع:
      Date Created: 20060725 Date Completed: 20060911 Latest Revision: 20061115
    • الموضوع:
      20250114
    • الرقم المعرف:
      10.1038/ng1850
    • الرقم المعرف:
      16862159