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Optimal production of poly-gamma-glutamic acid by metabolically engineered Escherichia coli.

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  • المؤلفون: Jiang H;Jiang H; Shang L; Yoon SH; Lee SY; Yu Z
  • المصدر:
    Biotechnology letters [Biotechnol Lett] 2006 Aug; Vol. 28 (16), pp. 1241-6. Date of Electronic Publication: 2006 Jul 01.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0141-5492 (Print) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE
    • بيانات النشر:
      Publication: 1999- : Dordrecht : Kluwer Academic Publishers
      Original Publication: [Kew, Eng., Science and Technology Letters]
    • الموضوع:
      Biotechnology/*methods ; Escherichia coli/*metabolism ; Polyglutamic Acid/*analogs & derivatives; Bacterial Proteins/chemistry ; DNA Primers/chemistry ; Gene Expression Regulation, Bacterial ; Genetic Vectors ; Models, Genetic ; Nitrogen/metabolism ; Oligonucleotide Array Sequence Analysis ; Plasmids/metabolism ; Polyglutamic Acid/chemistry ; Protein Engineering/methods ; Recombinant Proteins/chemistry ; Time Factors
    • نبذة مختصرة :
      Metabolically-engineered Escherichia coli strains were developed by cloning poly-gamma-glutamic acid (gamma-PGA) biosynthesis genes, consisting of pgsB, pgsC and pgsA, from Bacillus subtilis The metabolic and regulatory pathways of gamma-PGA biosynthesis in E. coli were analyzed by DNA microarray. The inducible trc promoter and a constitutive promoter (P(HCE)) derived from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toebii were employed. The constitutive HCE promoter was more efficient than inducible trc promoter for the expression of gamma-PGA biosynthesis genes. DNA microarray analysis showed that the expression levels of several NtrC family genes, glnA, glnK, glnG, yhdX, yhdY, yhdZ, amtB, nac, argT and cbl were up-regulated and sucA, B, C, D genes were down-regulated. When (NH(4))(2)SO(4 )was added at 40 g/l into the feeding solution, the final gamma-PGA concentration reached 3.7 g/l in the fed-batch culture of recombinant E. coli/pCOpgs.
    • الرقم المعرف:
      0 (Bacterial Proteins)
      0 (DNA Primers)
      0 (Recombinant Proteins)
      0 (poly(gamma-glutamic acid))
      25513-46-6 (Polyglutamic Acid)
      N762921K75 (Nitrogen)
    • الموضوع:
      Date Created: 20060704 Date Completed: 20070126 Latest Revision: 20131121
    • الموضوع:
      20231215
    • الرقم المعرف:
      10.1007/s10529-006-9080-0
    • الرقم المعرف:
      16816893