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Optimal production of poly-gamma-glutamic acid by metabolically engineered Escherichia coli.
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- المؤلفون: Jiang H;Jiang H; Shang L; Yoon SH; Lee SY; Yu Z
- المصدر:
Biotechnology letters [Biotechnol Lett] 2006 Aug; Vol. 28 (16), pp. 1241-6. Date of Electronic Publication: 2006 Jul 01.
- نوع النشر :
Journal Article; Research Support, Non-U.S. Gov't
- اللغة:
English
- معلومة اضافية
- المصدر:
Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0141-5492 (Print) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE
- بيانات النشر:
Publication: 1999- : Dordrecht : Kluwer Academic Publishers
Original Publication: [Kew, Eng., Science and Technology Letters]
- الموضوع:
- نبذة مختصرة :
Metabolically-engineered Escherichia coli strains were developed by cloning poly-gamma-glutamic acid (gamma-PGA) biosynthesis genes, consisting of pgsB, pgsC and pgsA, from Bacillus subtilis The metabolic and regulatory pathways of gamma-PGA biosynthesis in E. coli were analyzed by DNA microarray. The inducible trc promoter and a constitutive promoter (P(HCE)) derived from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toebii were employed. The constitutive HCE promoter was more efficient than inducible trc promoter for the expression of gamma-PGA biosynthesis genes. DNA microarray analysis showed that the expression levels of several NtrC family genes, glnA, glnK, glnG, yhdX, yhdY, yhdZ, amtB, nac, argT and cbl were up-regulated and sucA, B, C, D genes were down-regulated. When (NH(4))(2)SO(4 )was added at 40 g/l into the feeding solution, the final gamma-PGA concentration reached 3.7 g/l in the fed-batch culture of recombinant E. coli/pCOpgs.
- الرقم المعرف:
0 (Bacterial Proteins)
0 (DNA Primers)
0 (Recombinant Proteins)
0 (poly(gamma-glutamic acid))
25513-46-6 (Polyglutamic Acid)
N762921K75 (Nitrogen)
- الموضوع:
Date Created: 20060704 Date Completed: 20070126 Latest Revision: 20131121
- الموضوع:
20231215
- الرقم المعرف:
10.1007/s10529-006-9080-0
- الرقم المعرف:
16816893
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