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FTIR-spectral analysis of two photosynthetic hydrogen-producing [corrected] strains and their extracellular polymeric substances.

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  • المؤلفون: Sheng GP;Sheng GP; Yu HQ; Wang CM
  • المصدر:
    Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2006 Nov; Vol. 73 (1), pp. 204-10. Date of Electronic Publication: 2006 Jun 10.
  • نوع النشر :
    Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0175-7598 (Print) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin ; New York : Springer International, c1984-
    • الموضوع:
      Spectroscopy, Fourier Transform Infrared*; Biopolymers/*chemistry ; Bradyrhizobiaceae/*chemistry ; Rhodobacter capsulatus/*chemistry; Bacterial Proteins/analysis ; Carbohydrates/analysis ; Lipids/analysis ; Nucleic Acids/analysis
    • نبذة مختصرة :
      The Fourier transform infrared (FTIR) spectra of the cells of two photosynthetic H(2)-producing strains, Rhodoblastus acidophilus and Rhodobacter capsulatus, as well as their extracellular polymeric substances (EPS), were evaluated. The FTIR spectra of R. capsulatus and its EPS during its cultivation were also recorded. The main peaks in the spectra, including 1,080 cm(-1) (carbohydrates), 1,250 cm(-1) (nucleic acids), 2,830-2,930 cm(-1) (lipids), 1,660-1,535 cm(-1) (Amide I and II of proteins), were observed. The relative heights of these peaks in the spectra of the two strains were different, showing the difference in contents of various components in the cells or EPS. The ratios among the main components in the EPS obtained from the FTIR spectra were in good agreement with those from a conventional quantitative chemical analysis. As an easy, rapid, and direct technique, the FTIR spectroscopy could be used to characterize the components and their relative contents of EPS of photosynthetic bacteria.
    • Comments:
      Erratum in: Appl Microbiol Biotechnol. 2006 Nov;73(1):241.
    • الرقم المعرف:
      0 (Bacterial Proteins)
      0 (Biopolymers)
      0 (Carbohydrates)
      0 (Lipids)
      0 (Nucleic Acids)
    • الموضوع:
      Date Created: 20060613 Date Completed: 20070301 Latest Revision: 20061023
    • الموضوع:
      20221213
    • الرقم المعرف:
      10.1007/s00253-006-0442-2
    • الرقم المعرف:
      16767463