Effect of 5-hydroxytryptamine on neurogenic vasoconstriction in the isolated, autoperfused hindquarters of the rat.

Publisher: Wiley-Blackwell Country of Publication: Australia NLM ID: 0425076 Publication Model: Print Cited Medium: Print ISSN: 0305-1870 (Print) Linking ISSN: 03051870 NLM ISO Abbreviation: Clin Exp Pharmacol Physiol Subsets: MEDLINE

Publication: Oxford, England : Wiley-Blackwell
Original Publication: Oxford, Blackwell Scientific Publications.

Serotonin/*pharmacology ; Serotonin Agents/*pharmacology ; Vasoconstriction/*drug effects; 8-Hydroxy-2-(di-n-propylamino)tetralin/pharmacology ; Animals ; Biguanides/agonists ; Biphenyl Compounds/pharmacology ; Dose-Response Relationship, Drug ; Electric Stimulation ; Hindlimb/blood supply ; Hindlimb/drug effects ; Hindlimb/innervation ; In Vitro Techniques ; Infusions, Intravenous ; Male ; Methiothepin/pharmacology ; Oxadiazoles/pharmacology ; Perfusion ; Piperazines/pharmacology ; Pressure ; Quinoxalines/pharmacology ; Rats ; Rats, Wistar ; Ritanserin/pharmacology ; Serotonin/analogs & derivatives ; Serotonin 5-HT1 Receptor Agonists ; Serotonin 5-HT2 Receptor Agonists ; Serotonin 5-HT3 Receptor Agonists ; Serotonin Antagonists/pharmacology ; Serotonin Receptor Agonists/pharmacology ; Tryptamines/pharmacology

1. In the present study, we analysed the effect of different doses of 5-hydroxytryptamine (5-HT; intravenous infusions of 0.001-40 microg/kg per min) in the autoperfused hindquarters of the rat subjected to electrical stimulation (frequencies of 0.5-20 Hz) of the lumbar chains, investigating the relationship between the adrenergic and serotonergic systems in this vascular bed. 2. Because we observed that 5-HT inhibited the increases in perfusion pressure induced by electrical stimulation of the lumbar chains, we used different agonists and antagonists to analyse the mechanism of action of 5-HT. 3. The effect of 5-HT was inhibited by methiothepin (a non-specific 5-HT receptor antagonist), but not by ritanserin (a selective 5-HT2 receptor antagonist). The effects of 5-HT were mimicked by 5-carboxamidotryptamine (a 5-HT1 receptor agonist) and L-694 247 (a selective 5-HT1D receptor agonist), but not by 8-hydroxy-2-dipropylaminotetralin (a 5-HT1A receptor agonist), CGS-12066B (a 5-HT1B receptor agonist), alpha-methyl-5-HT (a 5-HT2 receptor agonist), 1-(3-chlorophenyl) piperazine (a 5-HT2C receptor agonist) or 1-phenylbiguanide (a 5-HT3 receptor agonist). The selective 5-HT1D/1B receptor antagonist BRL 15572 inhibited the effect of the agonist L-694 247. 4. Our data suggest that 5-HT inhibits the increases in perfusion pressure induced by the electrical stimulation of the lumbar chains, acting on presynaptic 5-HT1D receptors and decreasing the release of noradrenaline from the sympathetic nerves in the hindquarter vascular bed of the rat.

0 (Biguanides)
0 (Biphenyl Compounds)
0 (Oxadiazoles)
0 (Piperazines)
0 (Quinoxalines)
0 (Serotonin 5-HT1 Receptor Agonists)
0 (Serotonin 5-HT2 Receptor Agonists)
0 (Serotonin 5-HT3 Receptor Agonists)
0 (Serotonin Agents)
0 (Serotonin Antagonists)
0 (Serotonin Receptor Agonists)
0 (Tryptamines)
109028-10-6 (CGS 12066B)
137403-12-4 (L 694247)
145TFV465S (Ritanserin)
22965-81-7 (alpha-methylserotonin)
333DO1RDJY (Serotonin)
55D94103HL (Methiothepin)
78950-78-4 (8-Hydroxy-2-(di-n-propylamino)tetralin)
91H76044O0 (5-carboxamidotryptamine)
LY8CZE6XUD (BRL 15572)
REY0CNO998 (1-(3-chlorophenyl)piperazine)
W8PKA3T2I3 (phenyl biguanide)

Date Created: 20050922 Date Completed: 20060105 Latest Revision: 20171116

20240829

10.1111/j.1440-1681.2010.04281.x

16173953