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Study Cytotoxicity and Genotoxicity of Hemolysin Using Human Lymphocyte .
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- معلومة اضافية
- نبذة مختصرة :
The cytotoxic effects on human lymphocytes of Hemolysin sample (one pure sample produced in the laboratory for this study) were assessed at four different concentrations (25, 50, 100 and 200 μL) using the methylthiazol tetrazolium (MTT) bioassay. The results showed that growth was inhibited by 21.4, 32.3, 48.7, and 79.34 for each of the four concentrations of the pure sample, respectively, Deoxyribonucleic acid (DNA) was extracted from the lymphocytes and analysed by electrophoresis on a 1% agarose gel. Hemolysin appeared to have the ability to degrade or damage the DNA. The present study showed that both the growth inhibition and DNA damage experienced by the human lymphocytes increased linearly with increasing concentrations of the toxin. [ABSTRACT FROM AUTHOR]
- نبذة مختصرة :
Copyright of Egyptian Academic Journal of Biological Sciences, G, Microbiology is the property of Egyptian Academic Journal of Biological Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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