Characterization of the human SLC2A11 (GLUT11) gene: alternative promoter usage, function, expression, and subcellular distribution of three isoforms, and lack of mouse orthologue.

Publisher: Informa Healthcare Country of Publication: England NLM ID: 9430797 Publication Model: Print Cited Medium: Print ISSN: 0968-7688 (Print) Linking ISSN: 09687688 NLM ISO Abbreviation: Mol Membr Biol Subsets: MEDLINE

Publication: London : Informa Healthcare
Original Publication: London, UK : Taylor & Francis, c1994-2019.

Promoter Regions, Genetic*; Gene Expression Regulation/*physiology ; Glucose Transport Proteins, Facilitative/*genetics ; Glucose Transport Proteins, Facilitative/*metabolism; Alternative Splicing ; Animals ; COS Cells ; Chlorocebus aethiops ; Exons/genetics ; Glucose/metabolism ; Glucose Transport Proteins, Facilitative/biosynthesis ; Glucose Transport Proteins, Facilitative/physiology ; Humans ; Mice ; Organ Specificity/genetics ; Protein Isoforms/biosynthesis ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Protein Isoforms/physiology ; Protein Transport/genetics ; RNA, Messenger/genetics ; Rats ; Sequence Homology, Nucleic Acid ; Subcellular Fractions/metabolism ; Substrate Specificity/genetics ; Transcription, Genetic ; Xenopus laevis

GLUT11 (SLC2A11) is a class II sugar transport facilitator which exhibits highest similarity with the fructose transporter GLUT5 (about 42%). Here we demonstrate that separate exons 1 (exon 1A, exon 1B, and exon 1C) of the SLC2A11 gene generate mRNAs of three GLUT11 variants (GLUT11-A, GLUT11-B, and GLUT11-C) that differ in the amino acid sequence of their N-termini. All three 5'-flanking regions of exon 1A, exon 1B and exon 1C exhibited promoter activity when expressed as luciferase fusion constructs in COS-7 cells. 5'-RACE-PCR, quantitative real-time PCR, and Northern blot analysis performed with specific probes for exon 1A, 1B and 1C demonstrated that GLUT11-A is expressed in heart, skeletal muscle, and kidney, GLUT11-B in kidney, adipose tissue, and placenta, and GLUT11-C in adipose tissue, heart, skeletal muscle, and pancreas. Surprisingly, mice and rats lack the SLC2A11 gene. When expressed in Xenopus oocytes, all three GLUT11 isoforms transport glucose and fructose but not galactose. There was no apparent difference in the subcellular distribution of the three isoforms expressed in COS-7 cells. Our data indicate that different promoters and splicing of the human SLC2A11 gene generate three GLUT11 isoforms which are expressed in a tissue specific manner but do not appear to differ in their functional characteristics.

0 (Glucose Transport Proteins, Facilitative)
0 (Protein Isoforms)
0 (RNA, Messenger)
0 (SLC2A11 protein, human)
IY9XDZ35W2 (Glucose)

Date Created: 20050913 Date Completed: 20060714 Latest Revision: 20191210

20221213

10.1080/09687860500166143

16154905