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Dimerisation of the Yeast K + Translocation Protein Trk1 Depends on the K + Concentration.
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- المؤلفون: Kulik, Natalia1 (AUTHOR); Kale, Deepika1 (AUTHOR); Spurna, Karin1 (AUTHOR); Shamayeva, Katsiaryna1 (AUTHOR); Hauser, Fabian2 (AUTHOR); Milic, Sandra2 (AUTHOR); Janout, Hannah3,4 (AUTHOR); Zayats, Vasilina1 (AUTHOR); Jacak, Jaroslaw2 (AUTHOR); Ludwig, Jost1 (AUTHOR)
- المصدر:
International Journal of Molecular Sciences. Jan2023, Vol. 24 Issue 1, p398. 16p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
In baker's yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K+ uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel α subunit. Because most K-channels are proteins containing four channel-building α subunits, Trk1 could be functional as a monomer. However, related SKT proteins TrkH and KtrB were crystallised as dimers, and for Trk1, a tetrameric arrangement has been proposed based on molecular modelling. Here, based on Bimolecular Fluorescence Complementation experiments and single-molecule fluorescence microscopy combined with molecular modelling; we provide evidence that Trk1 can exist in the yeast plasma membrane as a monomer as well as a dimer. The association of monomers to dimers is regulated by the K+ concentration. [ABSTRACT FROM AUTHOR]
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