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Developmentally regulated mitochondrial biogenesis and cell death competence in maize pollen.
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- المؤلفون: Chamusco, Karen C.1 (AUTHOR); Milazzo, May N.2 (AUTHOR); Bhan, Kanchan S.3 (AUTHOR); Kamps, Terry L.1 (AUTHOR); Smith, Prestina4 (AUTHOR); Durojaiye, Modupeoluwa5 (AUTHOR); Moreira, Cristina D.6 (AUTHOR); Gallo, Maria7 (AUTHOR); Chase, Christine D.1 (AUTHOR)
- المصدر:
BMC Plant Biology. 11/1/2022, Vol. 22 Issue 1, p1-16. 16p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
Background: Cytoplasmic male sterility (CMS) is a maternally inherited failure to produce functional pollen that most commonly results from expression of novel, chimeric mitochondrial genes. In Zea mays, cytoplasmic male sterility type S (CMS-S) is characterized by the collapse of immature, bi-cellular pollen. Molecular and cellular features of developing CMS-S and normal (N) cytoplasm pollen were compared to determine the role of mitochondria in these differing developmental fates. Results: Terminal deoxynucleotidyl transferase dUTP nick end labeling revealed both chromatin and nuclear fragmentation in the collapsed CMS-S pollen, demonstrating a programmed cell death (PCD) event sharing morphological features with mitochondria-signaled apoptosis in animals. Maize plants expressing mitochondria-targeted green fluorescent protein (GFP) demonstrated dynamic changes in mitochondrial morphology and association with actin filaments through the course of N-cytoplasm pollen development, whereas mitochondrial targeting of GFP was lost and actin filaments were disorganized in developing CMS-S pollen. Immunoblotting revealed significant developmental regulation of mitochondrial biogenesis in both CMS-S and N mito-types. Nuclear and mitochondrial genome encoded components of the cytochrome respiratory pathway and ATP synthase were of low abundance at the microspore stage, but microspores accumulated abundant nuclear-encoded alternative oxidase (AOX). Cytochrome pathway and ATP synthase components accumulated whereas AOX levels declined during the maturation of N bi-cellular pollen. Increased abundance of cytochrome pathway components and declining AOX also characterized collapsed CMS-S pollen. The accumulation and robust RNA editing of mitochondrial transcripts implicated translational or post-translational control for the developmentally regulated accumulation of mitochondria-encoded proteins in both mito-types. Conclusions: CMS-S pollen collapse is a PCD event coincident with developmentally programmed mitochondrial events including the accumulation of mitochondrial respiratory proteins and declining protection against mitochondrial generation of reactive oxygen species. [ABSTRACT FROM AUTHOR]
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