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Nanozyme-based cascade SPR signal amplification for immunosensing of nitrated alpha-synuclein.
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- المؤلفون: Hu, Xiaojun1 (AUTHOR); Hu, Ruhui1 (AUTHOR); Zhu, Han1 (AUTHOR); Chen, Qiang1,2 (AUTHOR); Lu, Yongkai1 (AUTHOR); Chen, Jie1,2 (AUTHOR); Liu, Yawen2,3 (AUTHOR); Chen, Hongxia1 (AUTHOR)
- المصدر:
Microchimica Acta. Oct2022, Vol. 189 Issue 10, p1-11. 11p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
A self-assembled nanozyme of iron porphyrin mediated supramolecular modified gold nanoparticles (FpA) was fabricated to determine nitrated alpha-synuclein as the Tyr 39 residue (nT39 α-Syn) of a potential biomarker for early diagnosis of Parkinson's disease (PD). Mechanically, localized surface plasmon resonance (LSPR) and the mass effect caused by catalytic deposition of the nanozyme contributed to a cascade signal amplification strategy. The sensor allowed a signal amplification and selective nT39 α-Syn bioanalysis with a 1.34-fold enhancement by cascade amplified SPR signal and double specific recognition. The detection limit was 1.78 ng/mL in the detection range of 7–240 ng/mL. Benefiting from the excellent immunosensor, this method can distinguish healthy people and PD patients using actual samples. Overall, this strategy provides a nanozyme-based biosensing platform for the early diagnosis of PD and can be applied to detect other protein biomarkers, such as PD-L1. [ABSTRACT FROM AUTHOR]
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