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Protein-embedding technique: a potential approach to standardization of immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections.

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اقرأ أكثر حفظ في قائمتي
  • المؤلفون: Shi SR;Shi SR; Liu C; Perez J; Taylor CR
  • المصدر:
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society [J Histochem Cytochem] 2005 Sep; Vol. 53 (9), pp. 1167-70. Date of Electronic Publication: 2005 Jun 13.
  • نوع النشر :
    Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: SAGE Publications Country of Publication: United States NLM ID: 9815334 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0022-1554 (Print) Linking ISSN: 00221554 NLM ISO Abbreviation: J Histochem Cytochem Subsets: MEDLINE
    • بيانات النشر:
      Publication: <2011->: Thousand Oaks, CA : SAGE Publications
      Original Publication: Baltimore : Williams & Wilkins Co., c1953-
    • الموضوع:
      Fixatives* ; Formaldehyde* ; Paraffin Embedding*; Immunohistochemistry/*standards ; Proteins/*metabolism; Animals ; Antibodies, Monoclonal ; Goats ; Horses ; Keratins/immunology ; Keratins/metabolism ; Mice ; Microspheres ; Microtomy ; Polymers ; Reference Standards ; S100 Proteins/immunology ; S100 Proteins/metabolism
    • نبذة مختصرة :
      A serial study was performed to develop a protein-embedding technique for standardization of immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded (FFPE) tissue sections. A protein carrier matrix must have two phases, a liquid phase to allow uniform mixing of a protein and a solid phase forming a 'block' that can be fixed and processed in the same manner as human tissue. This standardized protein block would serve as a source of thin sections for control of IHC and therefore must also withstand the boiling conditions of antigen retrieval (AR). After multiple experiments, a method was developed utilizing polymer microsphere (beads) as a support medium for protein. The method showed particular promise for quantitative IHC.
    • Grant Information:
      1 R33 CA-103455-01 United States CA NCI NIH HHS
    • الرقم المعرف:
      0 (Antibodies, Monoclonal)
      0 (Fixatives)
      0 (Polymers)
      0 (Proteins)
      0 (S100 Proteins)
      1HG84L3525 (Formaldehyde)
      68238-35-7 (Keratins)
    • الموضوع:
      Date Created: 20050616 Date Completed: 20051018 Latest Revision: 20131121
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1369/jhc.5B6691.2005
    • الرقم المعرف:
      15956020