Bio-artificial periosteum for severe open fracture--an experimental study of osteogenic cell/collagen sponge composite as a bio-artificial periosteum.

Publisher: IOS Press Country of Publication: Netherlands NLM ID: 9104021 Publication Model: Print Cited Medium: Print ISSN: 0959-2989 (Print) Linking ISSN: 09592989 NLM ISO Abbreviation: Biomed Mater Eng Subsets: MEDLINE

Publication: Amsterdam : IOS Press
Original Publication: New York : Pergamon Press, c1991-

Fracture Healing*; Biocompatible Materials/*chemistry ; Biomedical Engineering/*methods ; Bone Substitutes/*chemistry ; Collagen/*chemistry ; Fractures, Open/*therapy ; Periosteum/*chemistry; Alkaline Phosphatase/metabolism ; Animals ; Bioartificial Organs ; Bone Marrow Cells/cytology ; Bone and Bones/diagnostic imaging ; Bone and Bones/ultrastructure ; In Vitro Techniques ; Male ; Microscopy, Electron, Scanning ; Osteocalcin/chemistry ; Osteocalcin/metabolism ; Osteogenesis ; Periosteum/diagnostic imaging ; Radiography ; Rats ; Rats, Inbred F344 ; Time Factors ; Wound Healing

In an attempt to reduce complications in cases of severe open fracture, we developed a bio-artificial periosteum composed of osteogenic cells and collagen sponge. In the present study, we evaluated the osteogenic potential of the bio-artificial periosteum in vivo and in vitro. After 4-week incubation in vitro, the bio-artificial periosteum had high alkaline phosphatase activity and osteocalcin content. Moreover, energy dispersive X-ray analysis revealed numerous crystal structures consisting of P and Ca on the surface of the bio-artificial periosteum. Using a rat model for severe bone injury, we examined the bone formation process in defect sites covered with the bio-artificial periosteum. New bone formation occurred in the central part of the bone defect as well as at the bone edge. We conclude that by using the bio-artificial periosteum, the fracture site benefited from an improved osteogenic environment. These results indicate that a clinical trial to further evaluate this technique should be conducted.

0 (Biocompatible Materials)
0 (Bone Substitutes)
104982-03-8 (Osteocalcin)
9007-34-5 (Collagen)
EC 3.1.3.1 (Alkaline Phosphatase)

Date Created: 20050525 Date Completed: 20050915 Latest Revision: 20161124

20231215

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