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Contribution of manganese peroxidase and laccase to dye decoloration by Trametes versicolor.

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  • المؤلفون: Champagne PP;Champagne PP; Ramsay JA
  • المصدر:
    Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2005 Dec; Vol. 69 (3), pp. 276-85. Date of Electronic Publication: 2005 Nov 15.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0175-7598 (Print) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin ; New York : Springer International, c1984-
    • الموضوع:
      Industrial Microbiology*; Basidiomycota/*metabolism ; Coloring Agents/*metabolism ; Laccase/*metabolism ; Peroxidases/*metabolism; Basidiomycota/enzymology ; Coloring Agents/chemistry ; Laccase/biosynthesis
    • نبذة مختصرة :
      During dye decoloration by Trametes versicolor ATCC 20869 in modified Kirk's medium, manganese peroxidase (MnP) and laccase were produced, but not lignin peroxidase, cellobiose dehydrogenase or manganese-independent peroxidase. Purified MnP decolorized azo dyes [amaranth, reactive black 5 (RB5) and Cibacron brilliant yellow] in Mn(2+)-dependent reactions but did not decolorize an anthraquinone dye [Remazol brilliant blue R (RBBR)]. However, the purified laccase decolorized RBBR five to ten times faster than the azo dyes and the addition of a redox mediator, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), did not alter decoloration rates. Amaranth and RB5 were decolorized the most rapidly by MnP since they have a hydroxyl group in an ortho position and a sulfonate group in the meta position relative to the azo bond. During a typical batch decoloration with the fungal culture, the ratio of laccase:MnP was 10:1 to 20:1 (based on enzyme activity) and increased to greater than 30:1 after decoloration was complete. Since MnP decolorized amaranth about 30 times more rapidly than laccase per unit of enzyme activity, MnP should have contributed more to decoloration than laccase in batch cultures.
    • الرقم المعرف:
      0 (Coloring Agents)
      EC 1.10.3.2 (Laccase)
      EC 1.11.1.- (Peroxidases)
      EC 1.11.1.13 (manganese peroxidase)
    • الموضوع:
      Date Created: 20050419 Date Completed: 20060209 Latest Revision: 20061115
    • الموضوع:
      20221213
    • الرقم المعرف:
      10.1007/s00253-005-1964-8
    • الرقم المعرف:
      15834615