Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Processing Request
Rate of Translation and Kinetics of Processing of Newly Synthesized Molecules of Two Major Outer-Membrane Proteins, the OmpA and OmpF Proteins, of Escherkhia coli K12.
Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Processing Request
- معلومة اضافية
- نبذة مختصرة :
The rate of synthesis of the OmpA and OmpF proteins, two of the major outer membrane proteins of Escherichia coli K12, was determined. At 25 °C both proteins were translated at 6.5 amino acids/s. and the OmpF protein was translated at 15 amino acids/s at 37 °. The former rate corresponded to a synthesis time of just over 50 s for both proteins, which is significantly faster than their reported rates of assembly into the outer membrane at 25 °C. The kinetics of processing of the pro-OmpF protein were also investigated in detail, and the pro-OmpF half-life estimated to be 3-5 s at 25 °C. However a fraction of the precursor was processed more slowly, which may explain the discrepancy between these data and our earlier published estimate of 30 s. Pro-OmpA protein was processed with similar kinetics. These results demonstrate that the rate-limiting step in the assembly of both proteins into the outer membrane is post-translational and follows the processing step. [ABSTRACT FROM AUTHOR]
- نبذة مختصرة :
Copyright of European Journal of Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
No Comments.