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A myxobacterial GH19 lysozyme with bacteriolytic activity on both Gram-positive and negative phytopathogens.
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- المؤلفون: Li, Yueqiu; Zhou, Xiaoli; Zhang, Xianjiao; Xu, Zhiqiang; Dong, Honghong; Yu, Guohui; Cheng, Ping; Yao, Qing; Zhu, Honghui
- المصدر:
AMB Express; 5/12/2022, Vol. 12 Issue 1, p1-10, 10p
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
Myxobacteria, as predatory bacteria, have good application potential in the biocontrol of pathogenic microorganisms. Extracellular enzymes are thought to play an important role in their predation and also provide resources for discovering new antibacterial molecules. We previously isolated a myxobacterium, Corallococcus silvisoli c25j21 GDMCC 1.1387, which is predatory to plant pathogenic bacteria. In this study, we identified an endolysin-like GH19 glycoside hydrolase, C25GH19B, from the genome of c25j21. After its heterologous expression and purification from E. coli, the enzymatic properties of C25GH19B were characterized. C25GH19B showed lysozyme activity with the optimal reaction conditions at 40 °C and pH 4.5–5.0. Moreover, C25GH19B showed bacteriolytic activity against both Gram-positive and Gram-negative plant pathogenic bacteria. Our research provides not only a candidate enzyme for the development of novel biocontrol agents but also an experimental basis for further study on the function and mechanisms of extracellular enzymes in myxobacterial predation. [ABSTRACT FROM AUTHOR]
- نبذة مختصرة :
Copyright of AMB Express is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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