Hepatocyte culture in bioartificial livers with different membrane characteristics.

Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Print Cited Medium: Print ISSN: 0141-5492 (Print) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE

Publication: 1999- : Dordrecht : Kluwer Academic Publishers
Original Publication: [Kew, Eng., Science and Technology Letters]

Bioreactors* ; Liver, Artificial* ; Membranes, Artificial*; Albumins/*biosynthesis ; Cell Culture Techniques/*methods ; Hepatocytes/*physiology ; Tissue Engineering/*methods ; Urea/*metabolism; Animals ; Biocompatible Materials/chemistry ; Cell Culture Techniques/instrumentation ; Cells, Immobilized/physiology ; Male ; Materials Testing ; Porosity ; Rats ; Rats, Sprague-Dawley ; Tissue Engineering/instrumentation

Rat hepatocytes were cultured in three polysulfone, hollow-fiber cartridges, characterized by two membrane variables: pore size and inner diameter (ID). Hepatocytes entrapped in a micro-filtration (MF) cartridge with the membrane pore size 0.1 microm had twice the production of urea and 4-fold the amount of albumin in comparison to the control cartridge, a ultra-filtration (UF) cartridge with a molecular weight cut-off (MWCO) of 100 kDa. Hepatocytes entrapped in a UF cartridge with ID of 0.5 mm secreted twice the amount of urea and 10-fold the amount of albumin compared with the control UF cartridge.

0 (Albumins)
0 (Biocompatible Materials)
0 (Membranes, Artificial)
8W8T17847W (Urea)

Date Created: 20041218 Date Completed: 20050505 Latest Revision: 20191210

20221213

10.1023/B:BILE.0000045641.81661.df

15604772