Fibroblast growth factor-1 improves cardiac functional recovery and enhances cell survival after ischemia and reperfusion: a fibroblast growth factor receptor, protein kinase C, and tyrosine kinase-dependent mechanism.

Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8301365 Publication Model: Print Cited Medium: Internet ISSN: 1558-3597 (Electronic) Linking ISSN: 07351097 NLM ISO Abbreviation: J Am Coll Cardiol Subsets: MEDLINE

Original Publication: [New York, N.Y.] : Elsevier Biomedical, [c1983-

Cell Survival/*physiology ; Fibroblast Growth Factor 1/*physiology ; Myocardial Ischemia/*physiopathology ; Recovery of Function/*physiology; Animals ; Blotting, Western ; Fibroblast Growth Factor 1/metabolism ; Heart Ventricles/metabolism ; Hemodynamics ; Immunohistochemistry ; In Vitro Techniques ; Male ; Mice ; Mice, Transgenic ; Myocardial Reperfusion ; Precipitin Tests ; Protein Kinase C/physiology ; Protein-Tyrosine Kinases/antagonists & inhibitors ; Protein-Tyrosine Kinases/physiology ; Pyrroles/pharmacology ; Receptors, Fibroblast Growth Factor/physiology ; Signal Transduction/physiology

Objectives: We sought to investigate the role of fibroblast growth factor (FGF)-1 during acute myocardial ischemia and reperfusion.
Background: The FGFs display cardioprotective effects during ischemia and reperfusion.
Methods: We investigated FGF-1-induced cardioprotection during ischemia and reperfusion and the intracellular signaling pathways responsible for these effects in an ex vivo murine setup of myocardial ischemia and reperfusion.
Results: Cardiac-specific human FGF-1 overexpression was associated with enhanced post-ischemic hemodynamic recovery and decreased lactate dehydrogenase release during reperfusion. Inhibition of the FGF receptor, protein kinase C (PKC), and tyrosine kinase (TK) resulted in blockade of FGF-1-induced protective effects on cardiac functional recovery and cell death.
Conclusions: The overexpression of FGF-1 induces cardioprotection through a pathway that involves the FGF receptor, PKC, and TK.

0 (Pyrroles)
0 (Receptors, Fibroblast Growth Factor)
0 (SU 5402)
104781-85-3 (Fibroblast Growth Factor 1)
EC 2.7.10.1 (Protein-Tyrosine Kinases)
EC 2.7.11.13 (Protein Kinase C)

Date Created: 20040901 Date Completed: 20040922 Latest Revision: 20220408

20240513

10.1016/j.jacc.2004.05.067

15337227