DYNAMIC THIOL/DISULFIDE HOMEOSTASIS AND OXIDANT STATUS IN PATIENTS WITH HYPOPARATHYROIDISM.

DINAMIČKA TIOL/DISULFIDNA HOMEOSTAZA I OKSIDACIONI STATUS KOD PACIJENATA SA HIPOPARATIROIDIZMOM. (Croatian)

Background: In this study, we aimed at determining the dynamic thiol/disulfide homeostasis and oxidant balance, and investigating the relation of these parameters to the severity of the disease and the serum calcium levels. Methods: 55 patients with iatrogenic hypoparathyroidism follow-ups and 40 healthy volunteers were included in the study. The blood dynamic thiol/sulfide balance, Total Antioxidant Status (TAS), Total Oxidant Status (EOS), Paraoxonase Enzyme Activity (PON) levels were measured in serum samples. Results: In our study, it was found that the disulfide, disul-fide/native thiol, disulfide/total thiol levels were higher in the hypoparathyroidism group. A negative correlation was found between 25-hydroxy vitamin D (25-OH vitamin D) and disulfide, disulfide/native thiol and disulfide/total thiol, and a positive correlation was found between native thiol and total thiol ratio; and the corrected calcium levels and PON levels were negatively correlated. Conclusions: Consequently, a change in favour of disulfide was found in the dynamic thiol-disulfide homeostasis in the hypoparathyroidism group in our study. [ABSTRACT FROM AUTHOR]

Uvod: Cilj ovog istraživanja je bio da utvrdimo dinamičnu tiol/disulfidnu homeostazu i ravnotežu oksidanata, i istražimo odnos ovih parametara prema težini bolesti i nivoima kalcijuma u serumu. Metode: U ovu studiju bilo je uključeno 55 pacijenata sa jatrogenim hipoparatiroidizmom i 40 zdravih dobrovoljaca. U uzorcima seruma određivana je dinamička ravnoteža tiola/sulfida u krvi, ukupni antioksidacioni status (TAS), ukupni oksidacioni status (TOS), aktivnost enzima paraok-sonaze (PON). Rezultati: U našoj studiji je utvrđeno da su nivoi disulfida, disulfida/prirodnog tiola, disulfida/ukupnog tiola bili viši u grupi disulfida/nativnog tiola i disulfida/ukupnog tiola, i utvrđena je pozitivna korelacija između odnosa prirodnog tiola i ukupnog tiola; a korigovani nivoi kalcijuma i nivoi PON bili su negativno povezani. Zaključak: Shodno tome, došlo je do promene u korist disulfida u dinamičkoj tiol disulfidnoj homeostazi u grupi hipoparatiroidizma u našoj studiji. [ABSTRACT FROM AUTHOR]

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