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Developing a new model system for potato genetics by androgenesis.
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- المؤلفون: Du, Miru1 (AUTHOR); Wang, Ting1 (AUTHOR); Lian, Qun2 (AUTHOR); Zhang, Xiaojie1 (AUTHOR); Xin, Guohui1 (AUTHOR); Pu, Yuanyuan1 (AUTHOR); Bryan, Glenn J.3 (AUTHOR); Qi, Jianjian1 (AUTHOR)
- المصدر:
Journal of Integrative Plant Biology. Apr2021, Vol. 63 Issue 4, p628-633. 6p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
High heterozygosity and tetrasomic inheritance complicate studies of asexually propagated polyploids, such as potato. Reverse genetics approaches, especially mutant library construction, can be an ideal choice if a proper mutagenesis genotype is available. Here, we aimed to generate a model system for potato research using anther cultures of Solanum verrucosum, a self‐compatible diploid potato with strong late blight resistance. Six of the 23 regenerants obtained (SVA4, SVA7, SVA22, SVA23, SVA32, and SVA33) were diploids, and their homozygosity was estimated to be >99.99% with 22 polymorphic InDel makers. Two lines—SVA4 and SVA32—had reduced stature (plant height ≤80 cm), high seed yield (>1,000 seeds/plant), and good tuber set (>30 tubers/plant). We further confirmed the full homozygosity of SVA4 and SVA32 using whole‐genome resequencing. These two regenerants possess all the characteristics of a model plant: diploidy, 100% homozygosity, self‐compatibility, and amenability to transgenesis. Thus, we have successfully generated two lines, SVA4 and SVA32, which can potentially be used for mutagenesis and as model plants to rejuvenate current methods of conducting potato research. [ABSTRACT FROM AUTHOR]
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