Evaluation of Upper Respiratory Tract Microbiota Changing with Chronic Cough in Children.

Objective: The role of the upper respiratory tract microbiota in the development of diseases is frequently investigated. The aim of this study was to determine the rates of the presence of Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aures, Pseudomonas aeruginosa, Moraxella catarrhalis, Klebsiella pneumoniae, and Prevotella bacteria in the upper respiratory tract microbiota in patients with chronic cough. It was also aimed to investigate the bacteria mentioned above. Method: It is a daily cough that lasts more than 4 weeks in children with chronic cough. These criteria shall be sought for the individuals to be sampled. A total of 120 upper respiratory tract samples from 60 patients with chronic cough and 60 healthy controls were collected and stored at -80° C. DNA was isolated from the stored samples using DNA isolation kits. Following DNA isolation, realtime-polymerase chain reaction (PCR) was performed on the DNA fragment encoding the 16S RNA using primers that were universal for all bacteria to determine the total number of bacteria in the sample. The main bacterial strains of the upper respiratory tract flora which were planned to be investigated later were Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aures, Pseudomonas aeruginosa, Moraxella catarrhalis, Klebsiella pneumniae, Prevotella spp. genus. As a result, the quantity of bacteria selected was quantitatively obtained. In order to find the percentage of the selected bacteria in the upper respiratory tract flora, the percentage of the selected bacterial amount was determined according to the total bacterial amount. These procedures were repeated for each different bacteria identified. By comparing the percentage slices of bacteria detected in the sample of patients and control group individuals, data were obtained about the effects of chronic cough on microbiotic change. Results: Benza(a)pyrene application was finalized and the sample collection process was completed and DNA isolation and PCR process are continuing. Sample collection was started on October 15th, 2019. Throat swab samples were taken from patients who were admitted to pediatric emergency, pediatric outpatient clinic and pediatric allergy outpatient clinics with chronic cough. Throat swab specimens were taken from the patients who applied to pediatric nephrology without control cough. Sample collection was completed from 120 patients, 60 from the control group and 60 from the patient group. DNA isolation and PCR procedures are in progress. [ABSTRACT FROM AUTHOR]