RainbowSTORM: an open-source ImageJ plug-in for spectroscopic single-molecule localization microscopy (sSMLM) data analysis and image reconstruction.

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المؤلفون: Davis, Janel L¹ (AUTHOR); Soetikno, Brian¹ (AUTHOR); Song, Ki-Hee¹ (AUTHOR); Zhang, Yang¹ (AUTHOR); Sun, Cheng² (AUTHOR); Zhang, Hao F¹ (AUTHOR)
المصدر:
Bioinformatics. Oct2020, Vol. 36 Issue 19, p4972-4974. 3p.
الموضوع:
*IMAGE analysis; *IMAGE reconstruction; *MICROSCOPY; *DATA analysis; *SINGLE molecules

معلومة اضافية
- الموضوع:
  MICROSOFT Windows (Operating system)
- نبذة مختصرة :
  Summary Spectroscopic single-molecule localization microscopy (sSMLM) simultaneously captures the spatial locations and full spectra of stochastically emitting fluorescent single molecules. It provides an optical platform to develop new multimolecular and functional imaging capabilities. While several open-source software suites provide subdiffraction localization of fluorescent molecules, software suites for spectroscopic analysis of sSMLM data remain unavailable. RainbowSTORM is an open-source ImageJ/FIJI plug-in for end-to-end spectroscopic analysis and visualization for sSMLM images. RainbowSTORM allows users to calibrate, preview and quantitatively analyze emission spectra acquired using different reported sSMLM system designs and fluorescent labels. Availability and implementation RainbowSTORM is a java plug-in for ImageJ (https://imagej.net)/FIJI (http://fiji.sc) freely available through: https://github.com/FOIL-NU/RainbowSTORM. RainbowSTORM has been tested with Windows and Mac operating systems and ImageJ/FIJI version 1.52. Supplementary information Supplementary data are available at Bioinformatics online. [ABSTRACT FROM AUTHOR]