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Hairy root induction of Papaver somniferum var. album, a difficult-to-transform plant, by A rhizogenes LBA 9402.

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  • معلومة اضافية
    • المصدر:
      Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0032-0935 (Print) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin, New York, Springer-Verlag [etc.]
    • الموضوع:
      Alkaloids/*biosynthesis ; Hypocotyl/*growth & development ; Papaver/*growth & development ; Plant Roots/*growth & development ; Plants, Genetically Modified/*growth & development ; Rhizobium/*growth & development; Benzophenanthridines ; Codeine/biosynthesis ; Culture Media ; Isoquinolines ; Morphine/metabolism ; Papaver/genetics ; Papaver/metabolism ; Plants, Genetically Modified/metabolism ; Rhizobium/genetics ; Stress, Mechanical ; Transformation, Genetic
    • نبذة مختصرة :
      Two strains of Agrobacterium rhizogenes (15834, LBA 9402) and one Agrobacterium tumefaciens strain [GV 3101 (PMP90RK, p35SGUS-2)] and four culture media were tested and compared for their ability to induce hairy root formation on wounded Papaver somniferum L. hypocotyls. Five weeks after the infection with A. rhizogenes LBA 9402, hairy roots appeared on 80% of the hypocotyls maintained in the hormone-free liquid medium. Six hairy-root cultures were established. Transformation was confirmed by polymerase chain reaction analysis. One clone was analysed for its alkaloid production. The total alkaloid content was higher in the transformed roots (0.46+/-0.06% DW) than in the untransformed roots (0.32+/-0.05% DW). The transformed roots accumulated three times more codeine (0.18+/-0.02% DW) than intact roots (0.05+/-0% DW). Moreover, morphine (0.255+/-0.03% DW) and sanguinarine (0.014+/-0% DW) were found in the liquid culture medium.
    • References:
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    • الرقم المعرف:
      0 (Alkaloids)
      0 (Benzophenanthridines)
      0 (Culture Media)
      0 (Isoquinolines)
      76I7G6D29C (Morphine)
      AV9VK043SS (sanguinarine)
      UX6OWY2V7J (Codeine)
    • الموضوع:
      Date Created: 20040124 Date Completed: 20040607 Latest Revision: 20220318
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1007/s00425-003-1196-z
    • الرقم المعرف:
      14740216