Item request has been placed!
×
Item request cannot be made.
×
Processing Request
ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8.
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
- المؤلفون: Kwon, S Chul1,2,3 (AUTHOR); Jang, Harim1,2 (AUTHOR); Shen, Siyuan4 (AUTHOR); Baek, S Chan1,2 (AUTHOR); Kim, Kijun1,2 (AUTHOR); Yang, Jihye1,2 (AUTHOR); Kim, Jeesoo1,2 (AUTHOR); Kim, Jong-Seo1,2 (AUTHOR); Wang, Suman4 (AUTHOR); Shi, Yunyu4 (AUTHOR); Li, Fudong4 (AUTHOR) ; Kim, V Narry1,2 (AUTHOR)
- المصدر:
Nucleic Acids Research. 11/4/2020, Vol. 48 Issue 19, p11097-11112. 16p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
The microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a new component of Microprocessor. Through a crystal structure and biochemical experiments, we reveal that ERH uses its hydrophobic groove to bind to a conserved region in the N-terminus of DGCR8, in a 2:2 stoichiometry. Knock-down of ERH or deletion of the DGCR8 N-terminus results in a reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases the processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144. Thus, the ERH dimer may mediate 'cluster assistance' in which Microprocessor is loaded onto a poor substrate with help from a high-affinity substrate in the same cluster. Our study reveals a role of ERH in the miRNA biogenesis pathway. [ABSTRACT FROM AUTHOR]
No Comments.