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การเพาะเลี้ยงเนื้อเยื่อจากเอ็มบริโอของบัวหลวงชมพูและบัวหลวงขาวและการตรวจสอบ ความไวของต้นกล้าต่อรังสีแกมมา (Thai)

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  • معلومة اضافية
    • Alternate Title:
      Embryo of Pink Lotus and White Lotus (Nelumbo Nucifera Gaertn.) tissue culture and determination of seedling sensitivity to gamma rays. (English)
    • نبذة مختصرة :
      Nelumbo Nucifera Gaertn. is a colorful water plant which has economic value and if mutates into a new species it's price will increase. This research aims to 1) culture tissues from embryos of Pink Lotus and White Lotus and 2) determine the sensitivity of their seedlings from tissue culture to gamma rays. Seeds of both Lotus varieties were sterilized with the sintering method. Then, their embryos were separated based on the standard color template RAL into 4 shades including Fern green, Yellow green, May green, and Leaf green. After culturing the embryos on MS agar for 8 weeks, the growth of seedlings was measured. The results showed no statistical significance of the petiole length and root number of the seedlings obtained from each shade of embryos. All seedlings had only 1 shoot/plant, therefore 3 mg/L BA broth was added onto MS agar. After further culturing for 8 weeks, the shoot numbers were increased in all plants. Then, the seedlings of both Lotus varieties were treated with different dosages of gamma rays at 0, 20, 30, and 40 Gy. The treated seedlings were cultured on MS agar for 4 weeks and their survival rate was determined. The probit curve analysis revealed that the LD50 of Pink Lotus and White Lotus were 36.99 and 35.34 Gy, respectively. [ABSTRACT FROM AUTHOR]
    • نبذة مختصرة :
      Copyright of Journal of Science & Technology MSU is the property of Mahasarakham University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)