Sugarcane genome architecture decrypted with chromosome‐specific oligo probes.

SUMMARY: Sugarcane (Saccharum spp.) is probably the crop with the most complex genome. Modern cultivars (2n = 100–120) are highly polyploids and aneuploids derived from interspecific hybridization between Saccharum officinarum (2n = 80) and Saccharum spontaneum (2n = 40–128). Chromosome‐specific oligonucleotide probes were used in combination with genomic in situ hybridization to analyze the genome architecture of modern cultivars and representatives of their parental species. The results validated a basic chromosome number of x = 10 for S. officinarum. In S. spontaneum, rearrangements occurred from a basic chromosome of x = 10, probably in the Northern part of India, in two steps leading to x = 9 and then x = 8. Each step involved three chromosomes that were rearranged into two. Further polyploidization led to the wide geographical extension of clones with x = 8. We showed that the S. spontaneum contribution to modern cultivars originated from cytotypes with x = 8 and varied in proportion between cultivars (13–20%). Modern cultivars had mainly 12 copies for each of the first four basic chromosomes, and a more variable number for those basic chromosomes whose structure differs between the two parental species. One−four of these copies corresponded to entire S. spontaneum chromosomes or interspecific recombinant chromosomes. In addition, a few inter‐chromosome translocations were revealed. The new information and cytogenetic tools described in this study substantially improve our understanding of the extreme level of complexity of modern sugarcane cultivar genomes. Significance Statement: Using fluorescence in situ hybridization with chromosome‐specific oligo probes, we developed karyotypes for the high polyploid sugarcane (2n = 100–120) and its two parental species. We described the chromosome rearrangements in Saccharum spontaneum, from an ancestral x = 10 shared with S. officinarum, that lead to x = 9 and x = 8. We showed that sugarcane cultivar genomes have, depending on chromosomes, various copy numbers with various numbers originating from the two parental species and included chromosomes with distinct basic architecture, interspecific recombinants and translocations. [ABSTRACT FROM AUTHOR]

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