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در بیماران تالاسمی دارای آلوآنتی بادی Rh ژنوتیپ سیستم گروه خونی 7931- درمانگاه تالاسمی بزرگسالان در سال های 79 (Persian)

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  • معلومة اضافية
    • Alternate Title:
      Rh blood group genotyping in alloimmunized thalassemic patients in Tehran Adult Thalassemia Clinic during 2017-18. (English)
    • الموضوع:
      IRAN
    • نبذة مختصرة :
      Background and Objectives The main antigens of the Rh blood group system (D,C,c,E,e) after the ABO system, are the most immune antigens. Due to existence of donor's red blood cells in the patient's circulation serologic methods can not accurately detect blood group antigens in patients with chronic blood transfusions, but molecular methods can overcome many of these limitations. Materials and Methods In this cross sectional descriptive study, peripheral blood samples were taken from two hundred of alloimmunized thalassemic patients including 81 (40.5%) male and 119 (59.5%) female with mean age of 30 ± 10.9 (age range 4-65) at Tehran Thalassemia Clinic during 1976-96. Phenotyping was done for C,c,E,e antigens. Sequence Specific Primers (SSPs)-PCR was performed and the discrepant results between the phenotype and genotyping were confirmed by DNA sequencing. Results In this study, the highest prevalence of alloantibodies in the Rh system pertained to Anti-E 22% (44 patients) and Anti-D 10% (20 patients) and the frequency of the alleles in this blood group was determined C 71% (142), c 72.5% (145), E 23% (46), e 98% (196). Thirty eight out of 200 patients had different results between serology and genotype. Discrepancies were 7.5% (14) (RHC), 6% (11) (RHc), 9.5% (17) (RHE), and 1.5% (3) (RHe). Conclusions Molecular methods can help to determine the exact antigens as a complementary method by solving the problems of the serological method. [ABSTRACT FROM AUTHOR]
    • نبذة مختصرة :
      Copyright of Scientific Journal of Iranian Blood Transfusion Organization is the property of Iranian Blood Transfusion Organization Research Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)