THE PERFORMANCE OF SEVEN MOLECULAR METHODS FOR THE DETECTION OF PRRSV.

KARAKTERISTIKE SEDAM RAZLIČITIH MOLEKULARNIH METODA ZA DETEKCIJU VIRUSA PRRS-A.

Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains. [ABSTRACT FROM AUTHOR]

Reproduktivni i respiratorni sindom svinja je virusno oboljenje svinja koje se karakteriše reproduktivnim poremećajima kod priplodnih životinja i respiratornim simptomima kod svih kategorija. U Srbiji je prvi slučaj PRRS-a otkriven 2001. godine nakon ilegalnog uvoza semena nerastova. PRRS se danas smatra ekonomski najznačajnijom bolešću svinja usled velikih direktnih i indirektnih gubitaka. Danas se za rutinsku dijagnostiku PRRS-a u zaraženim stadima koriste serološke metode (ELISA) i molekularne metode. Iako su savremene dijagnostičke tehnike veoma moćne, izuzetna raznolikost sojeva ovog virusa je često prepreka ka postavljanju pouzdane dijagnoze. U cilju utvrđivanja performansi sedam različitih metoda za detekciju PRRS virusa, koristili smo dvadeset uzoraka. Međutim, nijedna od metoda nije bila u stanju da otkrije sve PRRSV sojeve. Najbolja osetljivost je dobijena kombinovanjem dve metode. Treba naglasiti da ne postoji potpuno pouzdan test kojim se mogu dokazati svi sojevi virusa zbog čega je često potrebno kombinovati više testova ili metoda. [ABSTRACT FROM AUTHOR]